Copyright 2005 International Society of Musculoskeletal and Neuronal Interactions
Authors said: We constructed a 512 bp long RNA probe complementa- ry to a segment of 2745 bp long human TGF1 mRNA between 1486 and 1998 bases. This probe has 92% homolo- gy to the corresponding chicken TGF1 cDNA sequence and 73% homology to chicken TGF3 cDNA sequence24. We used the following set of primers:
forward primer: 5'-ATTTAGGTGACACTATATGAGGGCTTTCGCCTTAG-3'
reverse primer: 5'-TAATACGACTCACTATAGGGCGCACGCACGAT CATGTTGGAC-3'.
As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # JQ423909.1).