According to the authors, "The Sfrp-2 antisense probe was generated by linearising the full-length clone with HindIII and transcribing with T3 RNA
polymerase." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (NM_204773).
Authors indicated probe was 224 nt long. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_204773).
Authors indicate the probe was amplified by PCR using the following primers:
5_-ccgccatggcgcgccgcctc-3_ and 5_-ccaaagcttctaacactgcag-3_ and was extracted from NCBI Acc# NM_204773.1. Primer and sequence match is imperfect.