According to the authors, "A plasmid containing the entire coding
region of chicken Wnt10a was digested
with NsiI [ATGCA/T]followed by blunting
and was transcribed with T3 RNA
polymerase to prepare the antisense
probe." No NsaI site is identified in the current full length cDNA, so Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc#NM_001006590).