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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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TWIST2
twist family bHLH transcription factor 2

Gene

Official Symbol	TWIST2
Official Full Name	twist family bHLH transcription factor 2
CGNC ID
also known as	CDERMO-1, twist-related protein 2\|dermo, Twist2, Dermo1
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204679
Polypeptide	NP_990010

Gene Expression

In Situ Hybridization

GEISHA

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	117581	ENSG00000233608		MIM:607556
Mouse	13345	ENSMUSG00000007805	All phenotypic alleles (3):Targeted, knock-out(1) Targeted, other(2)	MGI:104685
Xenopus	100125350			5878699
Zebrafish	30395	ENSDARG00000078266	ZDB-GENE-980526-235	ZFIN:ZDB-GENE-980526-235

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395405
Ensembl Gene	ENSGALG00000004274
KEGG	gga:395405

Entry 1

GEISHA Id	TWIST2.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	GGACCAGTGAAGAGGAGTTGGAAAG
Reverse cDNA Template Primer	AATTAACCCTCACTAAAGGCACCCAGACAAACCACACACAAAAC	cDNA Template Length	749
Forward cDNA Template Probe	show TCGGAGCCCATACAACCACACGACGAACTATCAGGAATGTTGCTTCTCGCTTCTCTGTGATCAGTCACTTGGAGAGATG GTGCGAAGGTTCAGGCCCATAGCTTTGGTGCTGGCTCCTGCTCAGTCTAAGTGCTCTGCAAGAGTTCAGAGTGAGGGAG GTCAAGAGGGGGTCTTTTGGACCAGTTTACTTGAGGTCGTTAAGGTTTCAGAAGCTACAAACTTGGATACGTCCCAATT CCACTTCAGTCTCAGACACTGGACAGTCCTTTGGCGGCTGTGCTGGCCTCGCTCCGGCGGTGGCTAGTGTGAGGCCGAC ATGGACCACGCACCCTCCATCCTCCAGACTGAGAAGGCATAACTCAGCCTCTCGTGAGCCACGTAACTGCAACTCGTCA TCTTACTGTCCATCTCGTCGCTCTGTAGCACCTGGTAGAGGAAGTCTATATACCGCGCCGCAAGCTTGAGCGTCTGGAT TTTACTCAGTTTGTCAGAGGGCAACGTGGGGATGATTTTCCTCAGGGCGGCAAAAGCTTCGTTGAGCGACTGAGTCCTC TGCCTCTCTCTGACATTGGCCAGGATCCTCTGGCTCTGCAGTTCTTCATAAGACTGGGAGCTGGGACTGGACTTTTTTC CCCTCTTCCCTGGGTTGGGGCTGCCATCTTCGCTGGACTTCTTACTGTATCTTCTCTTCCTGCCAAATCTCTTTGGCTG CCTTTCCAACTCCTCTTCACAGGTACCAAA

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Area Pellucida
Hensen's Node

7-12

stage 8

stage 9

stage 10

stage 11

stage 12

Head Mesenchyme
Lateral Plate Mesoderm
Neural Plate/Tube
Somites

13-18

stage 17

stage 18

Face Mesenchyme
Leg
Neural Plate/Tube
Pharyngeal Arches and Clefts
Somites
Wing

19-24

stage 19

stage 20

Face Mesenchyme
Leg
Limb Buds
Lung
Neural Plate/Tube
Pharyngeal Arches and Clefts
Sclerotome
Somatic Mesoderm
Somites
Wing

stage 25

Face Mesenchyme
Leg Mesenchyme
Lung
Neural Plate/Tube
Pharyngeal Arches and Clefts
Somatic Mesoderm
Somites
Wing Mesenchyme

Entry 2

GEISHA Id	Twist2.Bothe.2006	Data Source	Publication
Complete cDNA Template Probe	show CGGTTTTTCCAAAGGATCTGTCTCAGGAATTTAAAGCACATTTAAGACACACACACTCACATAACATAAGGAGGGGGGG AAAGGCTCTTCCCCTCCCCTTCTTGCAGAAAGCATGGAAGAAAGCTCCAGTTCTCCTGTTTCCCCTGTGGATAGCTTGG GGACCAGTGAAGAGGAGTTGGAAAGGCAGCCAAAGAGATTTGGCAGGAAGAGAAGATACAGTAAGAAGTCCAGCGAAGA TGGCAGCCCCAACCCAGGGAAGAGGGGAAAAAAGTCCAGTCCCAGCTCCCAGTCTTATGAAGAACTGCAGAGCCAGAGG ATCCTGGCCAATGTCAGAGAGAGGCAGAGGACTCAGTCGCTCAACGAAGCTTTTGCCGCCCTGAGGAAAATCATCCCCA CGTTGCCCTCTGACAAACTGAGTAAAATCCAGACGCTCAAGCTTGCGGCGCGGTATATAGACTTCCTCTACCAGGTGCT ACAGAGCGACGAGATGGACAGTAAGATGACGAGTTGCAGTTACGTGGCTCACGAGAGGCTGAGTTATGCCTTCTCAGTC TGGAGGATGGAGGGTGCGTGGTCCATGTCGGCCTCACACTAGCCACCGCCGGAGCGAGGCCAGCACAGCCGCCAAAGGA CTGTCCAGTGTCTGAGACTGAAGTGGAATTGGGACGTATCCAAGTTTGTAGCTTCTGAAACCTTAACGACCTCAAGTAA ACTGGTCCAAAAGACCCCCTCTTGACCTCCCTCACTCTGAACTCTTGCAGAGCACTTAGACTGAGCAGGAGCCAGCACC AAAGCTATGGGCCTGAACCTTCGCACCATCTCTCCAAGTGACTGATCACAGAGAAGCGAGAAGCAACATTCCTGATAGT TCGTCGTTGTTGGGTTTTGTGTGTGGTTTGTCTGGGTGTTTGGTTTTAGTTCTTTTTTTTTTTTTTCTTTTCTCCTTGC TGAAAAAAAAAAAAAAGAATCTTAATTCAGGAACACATTTATGAGAAATCTTTGGGTTCGGATCTTACTTCCTCTTAGC CG
Citation	Bothe I, Dietrich S. The molecular setup of the avian head mesoderm and its implication for craniofacial myogenesis. Dev Dyn. 2006 Oct;235(10):2845-60.
Copyright	Copyright © 2006 Wiley-Liss, Inc.

Stage

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Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Mesoderm
Neural Plate/Tube
Notochord
Rhombomeres
Sclerotome
Somites

Entry 3

GEISHA Id	Dermo-1.Honig.2005	Data Source	Publication
Complete cDNA Template Probe	show TTTCCCCTGTGGATAGCTTGGGGACCAGTGAAGAGGAGTTGGAAAGGCAGCCAAAGAGATTTGGCAGGAAGAGAAGATA CAGTAAGAAGTCCAGCGAAGATGGCAGCCCCAACCCAGGGAAGAGGGGAAAAAAGTCCAGTCCCAGCTCCCAGTCTTAT GAAGAACTGCAGAGCCAGAGGATCCTGGCCAATGTCAGAGAGAGGCAGAGGACTCAGTCGCTCAACGAAGCTTTTGCCG CCCTGAGGAAAATCATCCCCACGTTGCCCTCTGACAAACTGAGTAAAATCCAGACGCTCAAGCTTGCGGCGCGGTATAT AGACTTCCTCTACCAGGTGCTACAGAGCGACGAGATGGACAGTAAGATGACGAGTTGCAGTTACGTGGCTCACGAGAGG CTGAGTTATGCCTTCTCAGTCTGGAGGATGGAGGGTGCGTGGTCCATGTCGGCCTCACACTAGCCACCGCCGGAGCGAG GCCAGCACAGCCGCCAAAGGACTGTCCAGTGTCTGAGACTGAAGTGGAATTGGGACGTATCCAAGTTTGTAGCTTCTGA AACCTTAACGACCTCAAGTAAACTGGTCCAAAAGACCCCCTCTTGACCTCCCTCACTCTGAACTCTTGCAGAGCACTTA GACTGAGCAGGAGCCAGCACCAAAGCTATGGGCCTGAACCTTCGCACCATCTCTCCAAGTGACTGATCACAGAGAAGCG AGAAGCA
Citation	Honig MG, Camilli SJ, Surineni KM, Knight BK, Hardin HM. The contributions of BMP4, positive guidance cues, and repulsive molecules to cutaneous nerve formation in the chick hindlimb. Dev Biol. 2005 Jun 1;282(1):257-73.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	According to the authors, "The cDermo-1 sequence (Scaal et al., 2001; GenBank accession number NM204679) was used to design primers that produce a 718-bp PCR product (nucleotides 138855; forward primer 5'-TTTCCCCTGTGGATAGCTTG-3'; reverse primer 5'-TGCTTCTCGCTTCTCTGTGA-3')." Information provided was used to obtain the sequence below from NCBI (NM_204679).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Wing Mesenchyme

subectodermal mesenchyme

Entry 4

GEISHA Id	TWIST2.Wade.2012	Data Source	Publication
Complete cDNA Template Probe	show `CATGGAAGAAAGCTCCAGTTCTCCTGTTTCCCCTGTGGATAGCTTGGGGACCAGTGAAGAGGAGTTGGAAAGGCAGCCA AAGAGATTTGGCAGGAAGAGAAGATACAGTAAGAAGTCCAGCGAAGATGGCAGCCCCAACCCAGGGAAGAGGGGAAAAA AGTCCAGTCCCAGCTCCCAGTCTTATGAAGAACTGCAGAGCCAGAGGATCCTGGCCAATGTCAGAGAGAGGCAGAGGAC TCAGTCGCTCAACGAAGCTTTTGCCGCCCTGAGGAAAATCATCCCCACGTTGCCCTCTGACAAACTGAGTAAAATCCAG ACGCTCAAGCTTGCGGCGCGGTATATAGACTTCCTCTACCAGGTGCTACAGAGCGACGAGATGGACAGTAAGATGACGA GTTGCAGTTACGTGGCTCACGAGAGGCTGAGTTATGCCTTCTCAGTCTGGAGGATGGAGGGTGCGTGGTCCATGTCGGC CTCACACTAG`
Citation	Wade C, Brinas I, Welfare M, Wicking C, Farlie PG. Twist2 contributes to termination of limb bud outgrowth and patterning through direct regulation of Grem1. Dev Biol. 2012 Oct 1;370(1):145-53.
Copyright	Copyright © 2012 Elsevier Inc. All rights reserved
Comments	The cDNA template used to generate the probe was obtained by PCR as described in the publication.