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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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EFNB2
ephrin B2

Gene

Official Symbol	EFNB2
Official Full Name	ephrin B2
CGNC ID	49409
also known as	ephrin-B2, ephrin-B2
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204824
Polypeptide	NP_990155
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.357

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	1948	ENSG00000125266		MIM:600527
Mouse	13642	ENSMUSG00000001300	All phenotypic alleles (17):Targeted, knock-out(2) Targeted, other(15)	MGI:105097
Xenopus	100101723, 100141484, 379645			488605
Zebrafish	30219, 114402	ENSDARG00000003783	ZDB-GENE-990415-67, ZDB-GENE-010618-1	ZFIN:ZDB-GENE-990415-67, ZFIN:ZDB-GENE-010618-1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	395625
Ensembl Gene
KEGG	gga:395625

Entry 1

GEISHA Id	EphrinB2.UAlinear	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 2

4-6

stage 4

stage 5

stage 6

Early Mesoderm
Lateral Plate Mesoderm
Primitive Streak

HH 5+

7-12

stage 7

stage 8

stage 10

stage 11

Forebrain
Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
Rhombomeres
Somites

13-18

stage 13

stage 14

stage 15

stage 16

Extraembryonic
Eye
Head Mesenchyme
Neural Crest
Neural Plate/Tube
Somites
Surface Ectoderm

Sections pending.

19-24

stage 20

stage 22

Eye
Forebrain
Head Mesenchyme
Lateral Plate Mesoderm
Leg
Limb Buds
Nasal Placode/Nerve
Wing

Sections pending.

stage 25

Eye
Forebrain
Head Mesenchyme
Leg
Midbrain
Spinal Cord
Wing

Sections pending.

Entry 2

GEISHA Id	EphrinB2.Baker.2003	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Baker RK, Antin PB. Ephs and ephrins during early stages of chick embryogenesis Dev Dyn. 2003 Sep;228(1):128-42.
Copyright	2003 Wiley-Liss, Inc.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

Lateral Plate Mesoderm
Mesoderm, early
Primitive Streak

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Aorta
Arteries
Blood Vessels
Dermatome
Diencephalon
Ear/Otic Placcode
Endoderm, early
Eye
Forebrain
Hindbrain
Lateral Plate Mesoderm
Mesoderm, early
Midbrain
Neural Crest
Neural Plate/Tube
Notochord
Primitive Streak
Somatopleure
Somites
Splanchnic Mesoderm
Tail
Telencephalon
Veins

vitelline artery vascular plexus

Entry 3

GEISHA Id	ephrin-B2.Mühleisen.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Mühleisen TW, Agoston Z, Schulte D. Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intraretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vivo. Dev Biol. 2006 Sep 1;297(1):59-73.
Copyright	Reprinted from Developmental Biology, Volume 297, Thomas W. Mühleisen, Zsuzsa Agoston and Dorothea Schulte, Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intaretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vitro, Pages 56-73, Copyright 2006, with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	According to the authors, "The cDNA clones used to generate in situ probes for EphA3, EphA4, ephrin-A5, ephrin-A2, EphB2, EphB3, ephrin-B1, ephrin-B2 or RCS, which recognizes a viral core protein, were described elsewhere (Schulte et al., 1999) or were gifts from J. Flanagan, C. Cepko (Harvard Medical School, Boston), or E. Pasquale (The Burnham Institute, La Jolla, California; Holash and Pasquale, 1995 and Cheng et al., 1995). As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204824)

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Eye
Retina

eye cup and neural retina

19-20

stage 20

Eye
Retina

eye cup and neural retina

Entry 4

GEISHA Id	EPHRIN-B2.Peters.2002	Data Source	Publication
Reverse cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Peters MA, Cepko CL (2002). The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments. Dev Biol. 2002 Nov 1;251(1):59-73.
Copyright	"Reprinted from Developmental Biology, 251(1), Peters MA, Cepko CL, The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments, 59-73, Copyright (2002), with permission from Elsevier". Copyright © ( 2002) Elsevier B.V. All rights reserved.
Comments	According to authors , "Probes for Pax2, Bmp4, Tbx5, SOHo, ephrin-B1, ephrin-B2, EphB1-B3, ALDH3/ALDH6, and ALDH1 were prepared as described (Refs)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_204824).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Hindbrain
Lens
Midbrain
Retina

double labeled

19-24

stage 22

Eye
Forebrain
Hindbrain
Midbrain
Pineal Gland

31-44

stage 44

Entry 5

GEISHA Id	EphrinB2.Davey.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Davey MG, James J, Paton IR, Burt DW, Tickle C. Analysis of talpid3 and wild-type chicken embryos reveals roles fro Hedgehog signalling in development of the limb bud vasculature. Dev Biol. 2007 Jan 1;301(1):155-65.
Copyright	Reprinted with permission from Elsevier. Copyright © 2007 Elsevier B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc #NM_204824.1).

Stage

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Location (click to highlight)

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stage 25

Arteries

subclavian artery

Entry 6

GEISHA Id	Ephrin-B2.Marin.2001	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Marín O, Blanco MJ, Nieto MA (2001). Differential expression of Eph receptors and ephrins correlates with the formation of topographic projections in primary and secondary visual circuits of the embryonic chick forebrain. Dev Biol. 2001 Jun 15;234(2):289-303.
Copyright	Copyright © 2001 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204824).

Stage

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Location (click to highlight)

Comments (click to highlight)

31-35

stage 35

Telencephalon

Entry 7

GEISHA Id	EFNB2.Hardy.2011	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Hardy KM, Yatskievych TA, Konieczka J, Bobbs AS, Antin PB. FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression. BMC Dev Biol. 2011 Mar 21;11:20.
Copyright	© 2011 Hardy et al; licensee BioMed Central Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204824).

Stage

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Location (click to highlight)

Comments (click to highlight)

stage 4

Early Mesoderm
Epiblast
Primitive Streak

lateral and medial mesoderm; lateral and preingression epiblast

Entry 8

GEISHA Id	EPHRINB2.Watanabe.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Watanabe T, Sato Y, Saito D, Tadokoro R, Takahashi Y. EphrinB2 coordinates the formation of a morphological boundary and cell epithelialization during somite segmentation. Proc Natl Acad Sci U S A. 2009 May 5; 106(18): 74677472.
Copyright	Copyright 2009 national Academy of Sciences USA
Comments	The probe was amplified by PCR using the following primers: 5′-ATGGCAGCGCGGCGGCGCGACG-3′ and 5′-CTAGTAAATGTTTGCTGGACTCTGAGG-3′ and was extracted from NCBI Acc#NM_204824.1.

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 12

Paraxial Mesoderm

Entry 9

GEISHA Id	EPHRINB2.Mellott.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGGCAGCGCGGCGGCGCGACGCCTCCGCCTGCAAGTACTGCTGGGGAGCCCTCATGGTTTTATGGAGAACTGCGCTGG CCAAGTCCATCGTTTTAGACCCCATCTATTGGAATTCCTCCAACCCCAAGTTCCTTCCTGGACAAGGATTGGTACTATA TCCACAGATAGGAGACAAACTGGATATTATATGCCCAAAGGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAG GTCTACATGGTTGATAAAGACCAAGCAGATAGCTGTGCTATTAGAAAGGACAATACACCTCTACTCAACTGTGCCAAGC CAGATCAAGATGTTAAGTTTACCATCAAATTTCAAGAATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAA AGATTATTACGTCATATCAACATCAAATGGGTCTTTGGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAA ACCATGAAGATCCTCATGAAAGTTGGACAAGATCCCAATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCC CTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCACCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGA TGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTCAGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATT TTCATCGTCATCATCATCACTTTGGTGGTTCTATTATTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACA CCACAACTCTCTCACTTAGTACATTAGCCACCCCAAAACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTAT CATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCCCCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTAC ATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAACATTTACTACAAGGTCTGA
Citation	Mellott DO, and Burke RD. Divergent roles for Eph and Ephrin in Avian Cranial Neural Crest. BMC Developmental Biology 2008, 8:56.
Copyright	© 2008 Mellott and Burke; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_204824.1.

Stage

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Location (click to highlight)

Comments (click to highlight)

13-14

stage 13

stage 14

Head Mesenchyme
Hindbrain

Entry 10

GEISHA Id	EFNB2.Anderson.2020	Data Source	Direct Submission
Complete cDNA Template Probe	show AATTCAGCCCTAATCTCTGGGGCCTGGAATTTCAGAAGAACAAAGATTATTACGTCATATCAACATCAAATGTGTCTTT GGAGGGCCTGAATAACCAGGAGGGAGGGGTGTGCCAGACAAAAACCATGAAGATCCTCATGAAAGTTGGACAAGATCCC AATTCTGCAGGGTTACCTCGGAGCACAGACCCAACCAAGCGCCCTGAACAGGAAGCTGGTACAAATGGGAAGAGCTCCA CCACGAGTCCCTTTGTGAAGGACCACTCAGGATCTAGCACAGATGGCAGTAAGGCTGGGCATTCCAGTATACTGGGTTC AGAAGTGGCCTTATTTGCAGGAATTGCATCAGGGTGCATCATTTTCATCGTCATCATCATCACTTTGGTGGTTCTATTA TTGAAGTACCGGAGAAGACACAGGAAGCATTCCCCACAACACACCACAACTCTCTCACTTAGTACATTAGCCACCCCAA AACGCAGTGGCAACAACAATGGTTCTGAGCCCAGTGACATTATCATTCCTCTAAGGACTGCAGACAGTGTTTTTTGCCC CCACTATGAAAAGGTCAGCGGCGACTATGGACATCCAGTGTACATAGTACAAGAGATGCCTCCTCAGAGTCCAGCAAAC ATCTACTACAAGGTCTAATCTAGAGCGGCCGCCACCGCGG
Comments	Images and probe sequence provided by Claire Anderson.