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A chicken embryo gene expression database

	Anatomical Location
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	Gene Name
	GEISHA ID
	Multiple Parameters

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RFNG
RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Gene

Official Symbol	RFNG
Official Full Name	RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
CGNC ID	2048
also known as	beta-1,3-N-acetylglucosaminyltransferase radical fringe\|O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, radical fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, Beta-1,3-N-acetylglucosaminyltransferase
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC

Sequence Information

Genomic
RNA	NM_204947
Polypeptide	NP_990278

Gene Expression

In Situ Hybridization

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly	40314			FBgn0011591
Human	5986	ENSG00000169733		MIM:602578
Mouse	19719	ENSMUSG00000025158	All phenotypic alleles (4):Targeted, knock-out(3) Targeted, other(1)	MGI:894275
Xenopus	100486895, 394282			483388
Zebrafish	414848	ENSDARG00000019746	ZDB-GENE-030131-5418	ZFIN:ZDB-GENE-030131-5418

Gene Ontology

Molecular Function	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity, metal ion binding
Biological Process	cell differentiation, nervous system development, pattern specification process
Cellular Component	integral component of Golgi membrane

Links to other databases

Entrez Gene	395789
Ensembl Gene	ENSGALG00000002841
KEGG	gga:395789

Entry 1

GEISHA Id	RFNG.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	ACAAAAGCAGGAGTCCAACGC
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGAGGAGGCTGAAAACAAGAACCC	cDNA Template Length	921
Forward cDNA Template Probe	show AATGGTTTTTTAAAAGTGTTTATATAGATACCAGTAATCTCCAGAGCTATGAATGACGTATCACTGAACCACAGGGACA TCTCAGTTCTATTGAGCAGAACACCTNATGGAGAGGTAGAACATCCCACCACCTACCCTGCCACGAGCCCTCCACGCTC TGTAACAGGCTGTCAGCATCACAGATGTTCCTGTTTTGAAACAACCACTGTGCTAGGTGGACTTTGATCCGAGTGCNTG CAGCACTCTTATTCCTGTGCCTCATGGTCAAAGCACTGCCTTAGCAGATAAAGGAAAAGAAATTTAAGAACAAGCTGCT GGATCGTAGCGAGTAGTCAGCTTGTTTGCTCCTCTATAAAATTAAATTAAATCATGCAAAGAGGACAATGTATCCAGTT GTTGAAAGGTTTGCTTCAAAAACAGAAAGCANAGAATATTATATTACAGGAGGCTGACACCTACACTGCAATTCATACT TGAATATGCTGCAGGTGTTTGCCTACATGGTACCACTTTTCAAATGGGAAGAAGTTAAGCAACTAGCTGAAATACACCG CTCAGCACAGCTGATAGAAATCTGAAACTTGCTAGGTCCGAATTCTTGCCATTAGTCATAGTTTCAGGTTATACAACAT CATGCTATTCTCCACTTAATTCAGCCTCAGCCTCTAGTACTAAACCTGCAAGTATTTTCTGAAGAGTTTGAGAGTAAAC AGTGGTTTTACAAAATCATTTTGTATGCTGTTCTTCACATCAGGTATAGATTGCACAAAGGCAAAGTTGGAAGTAACAA AACTAAACATAGAGAGTCTCAATTCTTCCACTTTCAAAACTAAGCTTATTTAATTTATAATGCAGCAGCAGGTGACATT GCTACAGCGTTGGATC

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 10

No staining stages 3-7

13-18

stage 16

19-24

stage 21

stage 22

25-26

stage 26

Entry 2

GEISHA Id	r-Fng.Ha.2003	Data Source	Publication
Complete cDNA Template Probe	show GGAGCCGTCTGGTGGAGGCCGCGGGGGAAACCGTCCGCGGGGCTCCCGGGGGCCATGAGGCTGGCCGCGCAGCGCGGCG GGGGCCGGTGGGGCCGCAACATGAGCAGCTCCTGCCTGGGGCTCCGCAGAGCCTGCTTCCTGCTGTCCGTCACCGCCGC CGCCGTTCTCCTGCTGCTGCTGCCCCGGGGACAGCCCCCCGCCGCGCCCCGCCGCCGCCCGCCGCCCGCCGGGCCCAGC AGGCCCTCCCCGAAGCGGGAGGCGCGGCCCGCGGGGAGCGACGTGCCCGGGGACCGCGGCGGCGGCTCGGGAGCCGCGG GGGGCGGCCGGGGCGTCGCCGGTAGCCCGTGGCCTTCGCGGAGGGTCCGCATGGGGCCTCCCGGCGGCTCGGCCAAGGA GAGCCTGGAGCTAAAAGACATCTTTATTGCGGTGAAAACGACGAGGAAGTATCACAAGACGCGGCTGGAGCTGCTGTTC CAAACCTGGATCTCCCGGGCGAGAGGACAGACATTCATATTCACAGACTGGGAGGATCGAGAGCTGCGCCTGAAAGCAG GGGATCATATGATCAACACTAACTGTTCTGCTGTCCACACCCGGCAAGCTCTGTGCTGCAAGATGTCTGTGGAATATGA TAAATTCCTAGAATCTGGACAAAAGTGGTTTTGCCATGTGGACGATGACAACTATGTGAATCCACGGACTCTCTTGCGT CTCTTATCTGCCTTCTCACCCAGCCAGGATGTCTATGTGGGACGACCGAGTCTGGACCATCCCATTGAAGCAGCTGACC ATGTCCAAAGCGATGGATCAAAGACAAGCGTGAAATTCTGGTTTGCCACAGGTGGAGCAGGGTTCTGTATCAGCAGAGG TCTTGCTCTGAAGATGAGTCCCTGGGCCAGCCTGGGTAATTTCATCAGTACTGCAGAAAGAGTGCGTCTTCCTGATGAC TGCACCATTGGCTACATCATTGAAGGGCTGCTGGAAGTAAAGCTGCTGCACAGCCCATTGTTCCATTCCCATCTGGAAA ATCTGCAGAGACTACAAGGAGAGTCTGTGCTGCAACAGGTAACCCTAAGTTATGGGGACCCTGAGAACAAACACAATGT TGTGAGTGTGGGAGGAGTGTTTGGACTTCAGCAAGACCCAACCAGATTTAAATCTGTCCATTGTCTTCTTTACCCTGAC ACTATTTGGTGCCCCAATAAGAAGATGTCATAACTTTTGACCAGTCATTGACACCTTTATCCTACCTACTTTGCGTAAA GCAAGAGTTGTGATGGGCTTTTTTTCTTCTGGGACACAAACAGACATATCTACAAAGGAGGTAGACTTTGTACAGAAGC AAGACTGGCTAATTATGGCAAGAAGGCATTTGTTCAGCTGCAGCCTGGGACATTGCCAAGAAGAAAATCTTCTATTTCT TGTTCTTTGGTCCAGTGGCTCTTCATGTGATGGCTCCAGTCATAGCTGTACAAGTCACTTTATGCTTTCATCTGATGTC ACATGAGCCCTGCCTATCATGTGAATCTGCGCTCAGATACATCCTAGGCAAATGCAGTACTTAGAATGATGGCATTCTT ACTACTGTTAGCAGCTTTCAGAGGCCATTGTCTTGAAGCTGGAATGTGTAAGCAGACTGCAGTCCCCATGGTGATGAGG GGGATGAAGTTTTTGCTTGTCTTTTTTGCGAACAGGACTTAAGAACTTCTGTGGCCTGCCATATTATACCTCCTCCACC TGTGAGCTGAAACACAGTCTGTTTGTAAACACCAGAAGTCCAGGAATTGCTAGGGTAGACAAGGGTGAAAGCCTTTGTC ATGGGAAAAACCCTGTGTAAGGGTAAACTGACTCAGGACTGCGCAAGAGTTGCTTACAGGGCACATTCTTCCCAGCAGT CTTTGTGTACCTCCCATGGAGGTGATTGTCAGACTTGGCACTCTTACCACTGAATGGCAGTGGCTGTTGATTATGATGG TGGTCTGCTAGGTCCAAGACTATTCCCTTGGTATTTGATACTGAAGATGTATTTGCCAGACTAGTTGGTAACTGTTTCT CCTCCTTTGCCCTCAGCAGGTTATATGAGCTAGAGCATGCCCATTGGCTGATGAAACACCCTGTTTTTGAAGCTGTGCT GTATCCTTCACTGCCTGCGAGGGAGGAGGAATGCTAGATAGCTGTAGTGCTGAGAAAGGTTTTGATGGTATCATTTCAG GTCTCTTAATTGTGTGCTTGTGGTTGCCCCACTTCAGCTTCTTAATGTAATCTTTTGAGACCAGTGTTTATTTTTACTC TACATCTTAATGTTTCAGAAAGTAATTCTGGCACTTGGTAAAAGGAAACTGGATGCTGATACAAAAGCAGGAGTCCAAC GCTGTAGCAATGTCACCTGCTGCTGCATTATAAATTAAATAAGCTTAGTTTTGAAAGTGGAAGAATTGAGACTCTCTAT GTTTAGTTTTGTTACTTCCAACTTTGCCTTTGTGCAATCTATACCTGATGTGAAGAACAGCATACAAAATGATTTTGTA AAACCACTGTTTACTCTCAAACTCTTCAGAAAATACTTGCAGGTTTAGTACTAGAGGCTGAGGCTGAATTAAGTGGAGA ATAGCATGATGTTGTATAACCTGAAACTATGACTAATGGCGAGAATTCGGACCGAGCAAGTTTCAGATTTCTATCAGCT GTGCTGAGCGGTGTATTTCAGCTAGTTGCTTAACTTCTTCCCATTTGAAAAGTGGTACCATGTAGGCAAACACCTGCAG CATATTCAAGTATGAAGTGCAGTGTAGGTGTCAGCCTCCTGTAATATAATATTCTCTGCTTTCTGTTTTTGAAGCAAAC CTTTCAACAACTGGATACGTTGTCCTCTTTGCATGATTTAATTTAATTTTATAGAGGAGCAAACAAGCTGACTACTCGC TACGATCCAGCAGCTTGTTCTTAAATTTCTTTTCCTTTATCTGCTAAGGCAGTGCTTTGACCATGAGGCACAGGAATAA GAGTGCTGCAAGCACTCGGATCAAAGTCCACCTAGCACAGTGGTTGTTTCAAAACAGGAACATCTGTGATGCTGACAGC CTGTTACAGAGCGTGGAGGGCTCGTGGCAGGGTAGGTGGTGGGATGTTCTACCTCTCCATCAGGTGGTCTGCTCAATAG AACTGAGATGTCCTGTGGTTCAGTGATACGTCATTCATAGCTCTGGAGATTACTGGTATCTATATAAACACTTTTTAAA AAACATTTGGTGGGTTCTTGTTTTCAGCCTCCTTGTAGCAAGGAGTTGTGAAGCTGCATAAGCACTTGATGAGATATCA TCCTCAGTGATCTCTGAATTCTGCTGGAGCTTTTCTTCCTCTCCTCTTAAGAGCAGCCTCCTGTCAGATACTATGGACA GCACAAGCTGGGTAGTGATTATGTGGCAAACTGCAGTTCAAAGATGACAACACTTCATACTGATGGCAGCAAAACTGCG TAGAAGTCCCATACTACAGTCATTTCCCACCCTAAATGGAGTTTGCTGCCTCCTTGTCATGCCTAATGCTGGTAGGGTG CTTATCAAGCTGAAAAAGGAACGTATTCTTCTGTAAAGCAATGCAAAAAAGCTTCTTTTAAGGTTTCTCTTGTTCACAA TACAGTAGGCTCTGTACATATACTGAGATGCAGCTTCTTAAACTACTGTCCCATACGTAAAACAATTCTATGAAATAAA TATTTAAGTATGTCAAAA
Citation	Ha AS, Riddle RD. cBlimp-1 expression in chick limb bud development. Gene Expr Patterns. 2003 Jun;3(3):297-300.
Copyright	Reprinted from Gene Expr Patterns, Volume 3, Issue 3, Alice S. Ha and Robert D. Riddle, cBlimp-1 expression in chick limb bud development, Pages 297-300, Copyright 2003, with permission from Elsevier. Copyright © 2003 Elsevier Science B.V. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # NM_204947).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Leg AER
Wing AER

Entry 3

GEISHA Id	RFNG.Tossell.2011	Data Source	Publication
Complete cDNA Template Probe	show GGAGCCGTCTGGTGGAGGCCGCGGGGGAAACCGTCCGCGGGGCTCCCGGGGGCCATGAGGCTGGCCGCGCAGCGCGGCG GGGGCCGGTGGGGCCGCAACATGAGCAGCTCCTGCCTGGGGCTCCGCAGAGCCTGCTTCCTGCTGTCCGTCACCGCCGC CGCCGTTCTCCTGCTGCTGCTGCCCCGGGGACAGCCCCCCGCCGCGCCCCGCCGCCGCCCGCCGCCCGCCGGGCCCAGC AGGCCCTCCCCGAAGCGGGAGGCGCGGCCCGCGGGGAGCGACGTGCCCGGGGACCGCGGCGGCGGCTCGGGAGCCGCGG GGGGCGGCCGGGGCGTCGCCGGTAGCCCGTGGCCTTCGCGGAGGGTCCGCATGGGGCCTCCCGGCGGCTCGGCCAAGGA GAGCCTGGAGCTAAAAGACATCTTTATTGCGGTGAAAACGACGAGGAAGTATCACAAGACGCGGCTGGAGCTGCTGTTC CAAACCTGGATCTCCCGGGCGAGAGGACAGACATTCATATTCACAGACTGGGAGGATCGAGAGCTGCGCCTGAAAGCAG GGGATCATATGATCAACACTAACTGTTCTGCTGTCCACACCCGGCAAGCTCTGTGCTGCAAGATGTCTGTGGAATATGA TAAATTCCTAGAATCTGGACAAAAGTGGTTTTGCCATGTGGACGATGACAACTATGTGAATCCACGGACTCTCTTGCGT CTCTTATCTGCCTTCTCACCCAGCCAGGATGTCTATGTGGGACGACCGAGTCTGGACCATCCCATTGAAGCAGCTGACC ATGTCCAAAGCGATGGATCAAAGACAAGCGTGAAATTCTGGTTTGCCACAGGTGGAGCAGGGTTCTGTATCAGCAGAGG TCTTGCTCTGAAGATGAGTCCCTGGGCCAGCCTGGGTAATTTCATCAGTACTGCAGAAAGAGTGCGTCTTCCTGATGAC TGCACCATTGGCTACATCATTGAAGGGCTGCTGGAAGTAAAGCTGCTGCACAGCCCATTGTTCCATTCCCATCTGGAAA ATCTGCAGAGACTACAAGGAGAGTCTGTGCTGCAACAGGTAACCCTAAGTTATGGGGACCCTGAGAACAAACACAATGT TGTGAGTGTGGGAGGAGTGTTTGGACTTCAGCAAGACCCAACCAGATTTAAATCTGTCCATTGTCTTCTTTACCCTGAC ACTATTTGGTGCCCCAATAAGAAGATGTCATAACTTTTGACCAGTCATTGACACCTTTATCCTACCTACTTTGCGTAAA GCAAGAGTTGTGATGGGCTTTTTTTCTTCTGGGACACAAACAGACATATCTACAAAGGAGGTAGACTTTGTACAGAAGC AAGACTGGCTAATTATGGCAAGAAGGCATTTGTTCAGCTGCAGCCTGGGACATTGCCAAGAAGAAAATCTTCTATTTCT TGTTCTTTGGTCCAGTGGCTCTTCATGTGATGGCTCCAGTCATAGCTGTACAAGTCACTTTATGCTTTCATCTGATGTC ACATGAGCCCTGCCTATCATGTGAATCTGCGCTCAGATACATCCTAGGCAAATGCAGTACTTAGAATGATGGCATTCTT ACTACTGTTAGCAGCTTTCAGAGGCCATTGTCTTGAAGCTGGAATGTGTAAGCAGACTGCAGTCCCCATGGTGATGAGG GGGATGAAGTTTTTGCTTGTCTTTTTTGCGAACAGGACTTAAGAACTTCTGTGGCCTGCCATATTATACCTCCTCCACC TGTGAGCTGAAACACAGTCTGTTTGTAAACACCAGAAGTCCAGGAATTGCTAGGGTAGACAAGGGTGAAAGCCTTTGTC ATGGGAAAAACCCTGTGTAAGGGTAAACTGACTCAGGACTGCGCAAGAGTTGCTTACAGGGCACATTCTTCCCAGCAGT CTTTGTGTACCTCCCATGGAGGTGATTGTCAGACTTGGCACTCTTACCACTGAATGGCAGTGGCTGTTGATTATGATGG TGGTCTGCTAGGTCCAAGACTATTCCCTTGGTATTTGATACTGAAGATGTATTTGCCAGACTAGTTGGTAACTGTTTCT CCTCCTTTGCCCTCAGCAGGTTATATGAGCTAGAGCATGCCCATTGGCTGATGAAACACCCTGTTTTTGAAGCTGTGCT GTATCCTTCACTGCCTGCGAGGGAGGAGGAATGCTAGATAGCTGTAGTGCTGAGAAAGGTTTTGATGGTATCATTTCAG GTCTCTTAATTGTGTGCTTGTGGTTGCCCCACTTCAGCTTCTTAATGTAATCTTTTGAGACCAGTGTTTATTTTTACTC TACATCTTAATGTTTCAGAAAGTAATTCTGGCACTTGGTAAAAGGAAACTGGATGCTGATACAAAAGCAGGAGTCCAAC GCTGTAGCAATGTCACCTGCTGCTGCATTATAAATTAAATAAGCTTAGTTTTGAAAGTGGAAGAATTGAGACTCTCTAT GTTTAGTTTTGTTACTTCCAACTTTGCCTTTGTGCAATCTATACCTGATGTGAAGAACAGCATACAAAATGATTTTGTA AAACCACTGTTTACTCTCAAACTCTTCAGAAAATACTTGCAGGTTTAGTACTAGAGGCTGAGGCTGAATTAAGTGGAGA ATAGCATGATGTTGTATAACCTGAAACTATGACTAATGGCGAGAATTCGGACCGAGCAAGTTTCAGATTTCTATCAGCT GTGCTGAGCGGTGTATTTCAGCTAGTTGCTTAACTTCTTCCCATTTGAAAAGTGGTACCATGTAGGCAAACACCTGCAG CATATTCAAGTATGAAGTGCAGTGTAGGTGTCAGCCTCCTGTAATATAATATTCTCTGCTTTCTGTTTTTGAAGCAAAC CTTTCAACAACTGGATACGTTGTCCTCTTTGCATGATTTAATTTAATTTTATAGAGGAGCAAACAAGCTGACTACTCGC TACGATCCAGCAGCTTGTTCTTAAATTTCTTTTCCTTTATCTGCTAAGGCAGTGCTTTGACCATGAGGCACAGGAATAA GAGTGCTGCAAGCACTCGGATCAAAGTCCACCTAGCACAGTGGTTGTTTCAAAACAGGAACATCTGTGATGCTGACAGC CTGTTACAGAGCGTGGAGGGCTCGTGGCAGGGTAGGTGGTGGGATGTTCTACCTCTCCATCAGGTGGTCTGCTCAATAG AACTGAGATGTCCTGTGGTTCAGTGATACGTCATTCATAGCTCTGGAGATTACTGGTATCTATATAAACACTTTTTAAA AAACATTTGGTGGGTTCTTGTTTTCAGCCTCCTTGTAGCAAGGAGTTGTGAAGCTGCATAAGCACTTGATGAGATATCA TCCTCAGTGATCTCTGAATTCTGCTGGAGCTTTTCTTCCTCTCCTCTTAAGAGCAGCCTCCTGTCAGATACTATGGACA GCACAAGCTGGGTAGTGATTATGTGGCAAACTGCAGTTCAAAGATGACAACACTTCATACTGATGGCAGCAAAACTGCG TAGAAGTCCCATACTACAGTCATTTCCCACCCTAAATGGAGTTTGCTGCCTCCTTGTCATGCCTAATGCTGGTAGGGTG CTTATCAAGCTGAAAAAGGAACGTATTCTTCTGTAAAGCAATGCAAAAAAGCTTCTTTTAAGGTTTCTCTTGTTCACAA TACAGTAGGCTCTGTACATATACTGAGATGCAGCTTCTTAAACTACTGTCCCATACGTAAAACAATTCTATGAAATAAA TATTTAAGTATGTCAAAA
Citation	Tossell K, Kiecker C, Wizenmann A, Lang E, Irving C. Notch signalling stabilises boundary formation at the midbrain-hindbrain organiser. Development. 2011 Sep;138(17):3745-57. Epub 2011 Jul 27.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_204947.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

stage 10

Neural Plate/Tube

Mouse

Emage	MGI:894275
MGI GXD	MGI:894275

Frog

Xenbase

Fruit Fly

BDGP	FBgn0011591

Zebra Finch

ZEBrA

Zebrafish

ZFIN	ZDB-GENE-030131-5418

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