Geisha

home

contact

A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

Quick Search

Data Class

Enter Text:

Gene Family QuickSearch

	Transcription Factors
	Growth Factors
	Receptors
	MicroRNAs

Human Disease Gene Search

	Downloads
	Protocols
	About GEISHA
	Contact Us

	Transgenic Birds
	Model Organism Databases
	Gene Expression Databases
	Genomic Resources
	Anatomical Atlases
	Chicken Stage Series

EFNA2
ephrin A2

Gene

Official Symbol	EFNA2
Official Full Name	ephrin A2
CGNC ID	49502
also known as	ELF-1, ephrin-A2\|EPH-related receptor tyrosine kinase ligand 6\|Eph ligand family-1\|LERK-6
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_204983
Polypeptide	NP_990314
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.2416

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human
Mouse
Xenopus
Zebrafish

Gene Ontology

Molecular Function
Biological Process	ephrin receptor signaling pathway
Cellular Component	anchored component of membrane, plasma membrane

Links to other databases

Entrez Gene	395831
Ensembl Gene
KEGG	gga:395831

Entry 1

GEISHA Id	EFNA2.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	TTATAAAAGCTTGCGGCCGCAGAATATACCGCTACGCCGTCTATTGG
Reverse cDNA Template Primer	GCTCTAGAAATTAACCCTCACTAAAGGAGTTGTTGCTGGTGAAGATGGG	cDNA Template Length	444
Forward cDNA Template Probe	show `AACGCAGCAACCCCAGGGTTCACCGGNGGGGGATTACACCGNTGGAGGTGAGCATCAATGACTACCTGGGACATCTACT GCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGC GTCCTGCGACCACCGGCAGAAGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTC TCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTG CGTCTCCCCCGAACGTGGTGGACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGA GTCCCCAGAGCCCATCTTCACCAGCAACAACTCCTTTAGTGAGGGTTAATTTCTAGAGCAAA`

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

stage 6

Early Mesoderm
Extraembryonic Mesoderm
Headfold
Lateral Plate Mesoderm
Neural Plate/Tube

7-12

stage 7

stage 9

stage 10

stage 11

stage 12

Head Mesenchyme
Headfold
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Somites

13-18

stage 17

stage 18

Head Mesenchyme
Lateral Plate Mesoderm
Midbrain
Wing

19-24

stage 20

stage 21

Head Mesenchyme
Limb Buds
Midbrain
Pharyngeal Arches and Clefts

stage 25

Head Mesenchyme
Leg Mesenchyme
Pharyngeal Arches and Clefts
Wing Mesenchyme

Entry 2

GEISHA Id	EphrinA2.Baker.2003	Data Source	Publication
Complete cDNA Template Probe	show CCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCGGCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCT CGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAA TGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATG GTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCC CCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCA CGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCG ACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCACCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTG CCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGGCTCTTAG
Citation	Baker RK, Antin PB. Ephs and ephrins during early stages of chick embryogenesis Dev Dyn. 2003 Sep;228(1):128-42.
Copyright	2003 Wiley-Liss, Inc.
Comments	EphrinA2 transcripts are present throughout the mesoderm (as determined from sectioned embryos, not shown) during stages 4-7; during this period, low levels of transcripts are also present in the primitive streak (Fig. 1A-C). Between stages 7 and 12, ephrinA2 is strongly expressed throughout the neurogenic ectoderm, particularly in the posterior half of the mesencephalon (the prospective midbrain). After stage 7, ephrinA2 expression continues in the head mesenchyme and cardiogenic mesoderm but is down-regulated in the mesoderm posterior to the heart-forming region.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

Area Pellucida
Epiblast
Mesoderm, early

area pellucida
early mesoderm

7-10

stage 7

stage 10

Heart
Mesoderm, early
Midbrain
Neural Plate/Tube
Primitive Streak

cardiogenic mesoderm
head mesenchyme
rostral mesoderm

Entry 3

GEISHA Id	ELF-1.Dube.2007	Data Source	Publication
Complete cDNA Template Probe	show CCGCCGCCCGCTGAGCCGCTCCGCTCCGCCGGGACGATGCCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCG GCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAG GTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCG CTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGA AGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTT CACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTG GACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCA CCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGG CTCTTAGTGCCGCGGGACGGACGGACGGGACGG
Citation	Dube A, Thai S, Gaspar J, Rudders S, Libermann TA, Iruela-Arispe L, Oettgen P. Elf-1 is a transcriptional regulator of the Tie2 gene during vascular development. Circ Res. 2001 Feb 2;88(2):237-44.
Copyright	Copyright © 2007 UBC Press
Comments	Authors said The cELF-1 cDNA fragment used to generate the probe is 700 bp in length and encodes the first 100 amino acids of the cELF-1 protein and 100 bp of the 5' untranslated region. The sequence listed above is NCBI Acc# NM_204983.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

31-36

stage 36

Blood Vessels
Heart Tube
Yolk Sack or Stalk

Entry 4

GEISHA Id	EPHRINA2.Takahashi.2003	Data Source	Publication
Complete cDNA Template Probe	show AGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCGGGG ATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGAGCG CATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAACGT TGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCTCTT TGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTGCCT CAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCACCAGCAACAACTCG TGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGGCT
Citation	Takahashi H, Shintani T, Sakuta H, Noda M. CBF1 controls the retinotectal topographical map along the anteroposterior axis through multiple mechanisms. Development. 2003 Nov;130(21):5203-15. Epub 2003 Sep 3.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors.
Comments	Authors indicated the probe was the 541 bp fragment of chick ephrin A2 (94-634; L40932).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-18

stage 18

Retina

19-24

stage 23

Retina

31-34

stage 34

Retina

Entry 5

GEISHA Id	EPHA2.Jayasena.2005	Data Source	Publication
Complete cDNA Template Probe	show `TGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAGGTTCCACCGCG GGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCGCTGCCCGCCGA GCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGAAGGGCTTCAAA CGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTTCACCCCCTTCT CTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTGGACAGACCCTG CCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTAT`
Citation	Jayasena CS, Flood WD, Koblar SA. High EphA3 expressing ophthalmic trigeminal sensory axons are sensitive to ephrin-A5-Fc: implications for lobe specific axon guidance. Neuroscience. 2005;135(1):97-109.
Copyright	Copyright © 2005 IBRO. Published by Elsevier Ltd. All rights reserved.
Comments	The complete cDNA template sequence was obtained from the information provided in the publication as described in Jayasena et al., 2005.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

13-15

stage 13

stage 15

Eye
Head Mesenchyme
Midbrain

mandibular and maxillary processes
mandibular process

all

Entry 6

GEISHA Id	ephrin‐A2.Iwamasa.1999	Data Source	Publication
Complete cDNA Template Probe	show CCGCCGCCCGCTGAGCCGCTCCGCTCCGCCGGGACGATGCCGCGCTGGGAGGCGGCCGCGCTGCTCGCCGCGATCGTCG GCGTCTGCGTGTGGAGCGACGACCCCGGCAAAGTGATCTCGGACCGCTACGCCGTCTATTGGAACCGCAGCAACCCCAG GTTCCACCGCGGGGATTACACCGTGGAGGTGAGCATCAATGACTACCTGGACATCTACTGCCCTCACTACGAGGAGCCG CTGCCCGCCGAGCGCATGGAGCGCTACGTCCTCTACATGGTCAACTACGAGGGCCACGCGTCCTGCGACCACCGGCAGA AGGGCTTCAAACGTTGGGAGTGCAACCGGCCCGACTCCCCCAGCGGACCCCTCAAGTTCTCAGAGAAGTTCCAGCTCTT CACCCCCTTCTCTTTGGGCTTCGAGTTCCGTCCCGGCCACGAGTATTACTACATCTCTGCGTCTCCCCTGAACGTGGTG GACAGACCCTGCCTCAAGCTGAAGGTTTACGTTCGGCCGACGAACGATTCCCTCTATGAGTCCCCAGAGCCCATCTTCA CCAGCAACAACTCGTGCTGCAGCCTTGCAGTGCCGCGTGCCGTGCTGGTGGCCGCGCCGGTCTTCTGGACTCTGCTGGG CTCTTAGTGCCGCGGGACGGACGGACGGGACGG
Citation	Iwamasa H, Ohta K, Yamada T, Ushijima K, Terasaki H, Tanaka H. Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles. Dev Growth Differ. 1999 Dec;41(6):685-98.
Copyright	Copyright © 1999 John Wiley & Sons, Inc.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_204983.1).