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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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EFNB1
ephrin B1

The human orthologue of this gene is associated
with the following human disease:

Craniofrontonasal dysplasia

Gene

Official Symbol	EFNB1
Official Full Name	ephrin B1
CGNC ID	49538
also known as	ephrin-B1\|CEK5 ligand\|CEK5-L, Ephrin-B1 precursor , CEK5 ligand, CEL5-L
gene type	protein-coding

Genomic Map

	Ensembl ID not known
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205035
Polypeptide	NP_990366
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.2142

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	1947	ENSG00000090776	MIM:304110	MIM:300035
Mouse	13641	ENSMUSG00000031217	All phenotypic alleles (15):Targeted, knock-out(3) Targeted, other(12)	MGI:102708
Xenopus	100494328, 394292			1011782
Zebrafish	114377	ENSDARG00000007723	ZDB-GENE-010618-2	ZFIN:ZDB-GENE-010618-2

Gene Ontology

Molecular Function
Biological Process	cell differentiation, nervous system development
Cellular Component	integral component of membrane

Links to other databases

Entrez Gene	395896
Ensembl Gene
KEGG	gga:395896

Entry 1

GEISHA Id	EphrinB1	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show AAGCGCTTTGAGTCGTCCGAGCCGCTGCCTGCGAAGGGGAGTAGAGGGGGCGGCACGGCGGGCGGCGGCGCTGTGCGAG CCGGGACGGCGGAGGGGACGCGCCGGAGCCGCCGTCGAGGCGAGAGGAGGAGCGGGAGGAACAGAAGGAGGAGGGCGGC GGCGGGGCGCTGCCCGCGGGCTGCGCGGCGCCGATGCGGGGCTGAGGAGCGGAGAGCCCCGCGGCATGGGCGCCTGACG CCCGGTGCCGTCCCGCCGCCGAGGGACGATGGCGCGGCCCCGCGGCGGGCGGTGGCTGCTGGGGGTGCTGCTCGCCCTG TGCCGCCTGGCCGCGCCGCTCGCCAAGAGCCTGGAGCCCGTCTCTTGGAGCGCCGGCAACCCCAAATTCATGAGTGGGA AAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCAAGCCTTACGA TTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGTGCTGGTGACG TGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGCCTGGAGTTCA AGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAGGAGGGGTCTG CCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCTGACGACGAGT CGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACGGTGGAGGAGC CCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCTCCAAAGTCGC CGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCTGATCAAGATC CGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCCAAATGCAGCG GGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGCACTACGAGAA GGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACATTTACTACAAG GTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCCCGGCCACGGC ACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTGTAGTTCTCAG CTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGCAGCGCGCACT CTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGGATTTCCCCTC GCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGGAGCCCTGGGA GGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAACCCGATTTACTACTAC TACTATTATTATTATTATAATTATTTTAATTTTTTTGTAACATTTTTATTTTTGTGAGTCGTGGCGGGACCCCCCGCCC TGGGCACATGCCACAGCCTCCTTCCAGCACGCCGTCCTGCTGGCGCAGGCAGATCCGCCGCTGCCGTTTTAAACTTTTG TGTTCGTAGCTGTTGACTTTTGTTTTAGTCTCTTTATAAAAA

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 9

stage 10

stage 11

stage 12

Anterior Intestinal Portal
Diencephalon
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Primitive Streak
Somites

13-18

stage 18

Eye
Midbrain
Pharyngeal Arches and Clefts
Somites

Entry 2

GEISHA Id	EphrinB1.Baker.2003	Data Source	Publication
Complete cDNA Template Probe	show GACGCCCGGTGCCGTCCCGCCGCCGAGGGACGATGGCGCGGCCCCGCGGCGGGCGGTGGCTGCTGGGGGTGCTGCTCGC CCTGTGCCGCCTGGCCGCGCCGCTCGCCAAGAGCCTGGAGCCCGTCTCTTGGAGCGCCGGCAACCCCAAATTCATGAGT GGGAAAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCAAGCCTT ACGATTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGTGCTGGT GACGTGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGCCTGGAG TTCAAGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAGGAGGGG TCTGCCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCTGACGAC GAGTCGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACGGTGGAG GAGCCCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCTCCAAAG TCGCCGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCTGATCAA GATCCGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCCAAATGC AGCGGGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGCACTACG AGAAGGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACATTTACTA CAAGGTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCCCGGCCA CGGCACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTGTAGTTC TCAGCTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGCAGCGCG CACTCTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGGATTTCC CCTCGCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGGAGCCCT GGGAGGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAAC
Citation	Baker RK, Antin PB. Ephs and ephrins during early stages of chick embryogenesis Dev Dyn. 2003 Sep;228(1):128-42.
Copyright	2003 Wiley-Liss, Inc.

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Blood Vessels
Endoderm, early
Extraembryonic
Head Process
Hensen's Node
Lateral Plate Mesoderm
Mesoderm, early
Notochord
Primitive Streak

7-12

stage 7

stage 8

stage 9

stage 10

stage 11

stage 12

Anterior Intestinal Portal
Aorta
Arteries
Blood Vessels
Cardinal Veins
Cardiovascular
Endoderm
Endoderm, early
Extraembryonic
Forebrain
Foregut
Head Process
Heart Tube
Hindbrain
Intermediate Mesoderm
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Notochord
Paraxial Mesoderm
Rhombomeres
Somites
Spinal Cord
Splanchnic Mesoderm
Tail
Veins

angioblasts

Entry 3

GEISHA Id	ephrin-B1.Mühleisen.2006	Data Source	Publication
Complete cDNA Template Probe	show AAGCGCTTTGAGTCGTCCGAGCCGCTGCCTGCGAAGGGGAGTAGAGGGGGCGGCACGGCGGGCGGCGGCGCTGTGCGAG CCGGGACGGCGGAGGGGACGCGCCGGAGCCGCCGTCGAGGCGAGAGGAGGAGCGGGAGGAACAGAAGGAGGAGGGCGGC GGCGGGGCGCTGCCCGCGGGCTGCGCGGCGCCGATGCGGGGCTGAGGAGCGGAGAGCCCCGCGGCATGGGCGCCTGACG CCCGGTGCCGTCCCGCCGCCGAGGGACGATGGCGCGGCCCCGCGGCGGGCGGTGGCTGCTGGGGGTGCTGCTCGCCCTG TGCCGCCTGGCCGCGCCGCTCGCCAAGAGCCTGGAGCCCGTCTCTTGGAGCGCCGGCAACCCCAAATTCATGAGTGGGA AAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCAAGCCTTACGA TTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGTGCTGGTGACG TGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGCCTGGAGTTCA AGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAGGAGGGGTCTG CCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCTGACGACGAGT CGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACGGTGGAGGAGC CCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCTCCAAAGTCGC CGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCTGATCAAGATC CGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCCAAATGCAGCG GGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGCACTACGAGAA GGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACATTTACTACAAG GTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCCCGGCCACGGC ACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTGTAGTTCTCAG CTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGCAGCGCGCACT CTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGGATTTCCCCTC GCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGGAGCCCTGGGA GGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAACCCGATTTACTACTAC TACTATTATTATTATTATAATTATTTTAATTTTTTTGTAACATTTTTATTTTTGTGAGTCGTGGCGGGACCCCCCGCCC TGGGCACATGCCACAGCCTCCTTCCAGCACGCCGTCCTGCTGGCGCAGGCAGATCCGCCGCTGCCGTTTTAAACTTTTG TGTTCGTAGCTGTTGACTTTTGTTTTAGTCTCTTTATAAAAA
Citation	Mühleisen TW, Agoston Z, Schulte D. Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intraretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vivo. Dev Biol. 2006 Sep 1;297(1):59-73.
Copyright	Reprinted from Developmental Biology, Volume 297, Thomas W. Mühleisen, Zsuzsa Agoston and Dorothea Schulte, Retroviral misexpression of cVax disturbs retinal ganglion cell axon fasciculation and intaretinal pathfinding in vivo and guidance of nasal ganglion cell axons in vitro, Pages 56-73, Copyright 2006, with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	According to the authors, "The cDNA clones used to generate in situ probes for EphA3, EphA4, ephrin-A5, ephrin-A2, EphB2, EphB3, ephrin-B1, ephrin-B2 or RCS, which recognizes a viral core protein, were described elsewhere (Schulte et al., 1999) or were gifts from J. Flanagan, C. Cepko (Harvard Medical School, Boston), or E. Pasquale (The Burnham Institute, La Jolla, California; Holash and Pasquale, 1995 and Cheng et al., 1995). As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205035.)

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 15

Eye
Retina

eye cup and neural retina

19-20

stage 20

Eye
Retina

eye cup and neural retina

Entry 4

GEISHA Id	EPHRIN-B1.Peters.2002	Data Source	Publication
Complete cDNA Template Probe	show AAGCGCTTTGAGTCGTCCGAGCCGCTGCCTGCGAAGGGGAGTAGAGGGGGCGGCACGGCGGGCGGCGGCGCTGTGCGAG CCGGGACGGCGGAGGGGACGCGCCGGAGCCGCCGTCGAGGCGAGAGGAGGAGCGGGAGGAACAGAAGGAGGAGGGCGGC GGCGGGGCGCTGCCCGCGGGCTGCGCGGCGCCGATGCGGGGCTGAGGAGCGGAGAGCCCCGCGGCATGGGCGCCTGACG CCCGGTGCCGTCCCGCCGCCGAGGGACGATGGCGCGGCCCCGCGGCGGGCGGTGGCTGCTGGGGGTGCTGCTCGCCCTG TGCCGCCTGGCCGCGCCGCTCGCCAAGAGCCTGGAGCCCGTCTCTTGGAGCGCCGGCAACCCCAAATTCATGAGTGGGA AAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCAAGCCTTACGA TTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGTGCTGGTGACG TGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGCCTGGAGTTCA AGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAGGAGGGGTCTG CCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCTGACGACGAGT CGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACGGTGGAGGAGC CCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCTCCAAAGTCGC CGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCTGATCAAGATC CGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCCAAATGCAGCG GGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGCACTACGAGAA GGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACATTTACTACAAG GTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCCCGGCCACGGC ACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTGTAGTTCTCAG CTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGCAGCGCGCACT CTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGGATTTCCCCTC GCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGGAGCCCTGGGA GGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAACCCGATTTACTACTAC TACTATTATTATTATTATAATTATTTTAATTTTTTTGTAACATTTTTATTTTTGTGAGTCGTGGCGGGACCCCCCGCCC TGGGCACATGCCACAGCCTCCTTCCAGCACGCCGTCCTGCTGGCGCAGGCAGATCCGCCGCTGCCGTTTTAAACTTTTG TGTTCGTAGCTGTTGACTTTTGTTTTAGTCTCTTTATAAAAA
Citation	Peters MA, Cepko CL (2002). The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments. Dev Biol. 2002 Nov 1;251(1):59-73.
Copyright	"Reprinted from Developmental Biology, 251(1), Peters MA, Cepko CL, The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments, 59-73, Copyright (2002), with permission from Elsevier". Copyright © ( 2002) Elsevier B.V. All rights reserved.
Comments	According to authors , "Probes for Pax2, Bmp4, Tbx5, SOHo, ephrin-B1, ephrin-B2, EphB1-B3, ALDH3/ALDH6, and ALDH1 were prepared as described (Refs)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205035).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 19

stage 22

Midbrain
Pharyngeal Arches and Clefts
Retina

31-44

stage 44

Entry 5

GEISHA Id	EFNB1.Bobak.2009	Data Source	Publication
Complete cDNA Template Probe	show AAGCGCTTTGAGTCGTCCGAGCCGCTGCCTGCGAAGGGGAGTAGAGGGGGCGGCACGGCGGGCGGCGGCGCTGTGCGAG CCGGGACGGCGGAGGGGACGCGCCGGAGCCGCCGTCGAGGCGAGAGGAGGAGCGGGAGGAACAGAAGGAGGAGGGCGGC GGCGGGGCGCTGCCCGCGGGCTGCGCGGCGCCGATGCGGGGCTGAGGAGCGGAGAGCCCCGCGGCATGGGCGCCTGACG CCCGGTGCCGTCCCGCCGCCGAGGGACGATGGCGCGGCCCCGCGGCGGGCGGTGGCTGCTGGGGGTGCTGCTCGCCCTG TGCCGCCTGGCCGCGCCGCTCGCCAAGAGCCTGGAGCCCGTCTCTTGGAGCGCCGGCAACCCCAAATTCATGAGTGGGA AAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCAAGCCTTACGA TTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGTGCTGGTGACG TGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGCCTGGAGTTCA AGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAGGAGGGGTCTG CCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCTGACGACGAGT CGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACGGTGGAGGAGC CCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCTCCAAAGTCGC CGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCTGATCAAGATC CGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCCAAATGCAGCG GGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGCACTACGAGAA GGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACATTTACTACAAG GTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCCCGGCCACGGC ACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTGTAGTTCTCAG CTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGCAGCGCGCACT CTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGGATTTCCCCTC GCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGGAGCCCTGGGA GGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAACCCGATTTACTACTAC TACTATTATTATTATTATAATTATTTTAATTTTTTTGTAACATTTTTATTTTTGTGAGTCGTGGCGGGACCCCCCGCCC TGGGCACATGCCACAGCCTCCTTCCAGCACGCCGTCCTGCTGGCGCAGGCAGATCCGCCGCTGCCGTTTTAAACTTTTG TGTTCGTAGCTGTTGACTTTTGTTTTAGTCTCTTTATAAAAA
Citation	Bobak N, Agoston Z, Schulte D. Evidence against involvement of Bmp receptor 1b signaling in fate specification of the chick mesencephalic alar plate at HH16. Neurosci Lett. 2009 Sep 25;461(3):223-8. Epub 2009 Jun 16
Copyright	Reprinted with permission from Elsevier. Copyright © 2009 Elsevier B.V. All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_205035.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-16

stage 16

Mesencephalon
Midbrain

Entry 6

GEISHA Id	EPHRINB1.Mellott.2008	Data Source	Publication
Complete cDNA Template Probe	show ATGAGTGGGAAAGGGTTGGTCATCTACCCGGAGATTGGGGACAAACTGGACATTATCTGCCCCAAGGCGGAGCCGTCCA AGCCTTACGATTACTACAAGCTGTACCTGGTGAAAAAGGACCAGGCAGATGCCTGCAGCACCGTCATGGACCCCAACGT GCTGGTGACGTGCAACCGGCCCGAGCAGGAGATCCGCTTCACCATCAAGTTTCAGGAGTTCAGCCCCAACTACATGGGC CTGGAGTTCAAGCGGCAGCAGGATTACTTCATCACATCCACCTCTAATGGGACACTGGATGGCCTGGAGAACCGGGAAG GAGGGGTCTGCCAGACACGCTCCATGAAGATCGTCATGAAAGTGGGGCAGGATCCAAACGCGGTGATCCCGGAGCAGCT GACGACGAGTCGGCCCAGCAAGGAGGCAGACAACACCGTGAAGATTGTGACGCAGAGCCCACGCCACAAAGTCCCCACG GTGGAGGAGCCCGGCAAGCCAGGTTCCGTCAACCAGAACGGCCAGGAGACCCAAGGTCCCAGCGATGGGTTCCTGAGCT CCAAAGTCGCCGTTTTCGCTGCCATCGGCGCGGGCTGCGTCATCTTCATCCTCATCATCATCTTCCTCGTCGTCCTCCT GATCAAGATCCGCAAGCGGCACCGGAAGCACACGCAGCAGCGGGCCGCAGCCTTGTCCCTCAGCACCTTGGCCAGCCCC AAATGCAGCGGGAACGCCGGCTCAGAGCCCAGCGACATCATCATCCCCTTACGGACTACAGAGAACAACTACTGCCCGC ACTACGAGAAGGTGAGCGGGGACTACGGGCACCCCGTCTACATCGTGCAGGAGATGCCCCCACAGAGCCCAGCCAACAT TTACTACAAGGTGTGAGGAGCAGCCTGGGCACGCCACGCAGGAGCGCCCGGTGGGGCCGGCAGCGGGAGGCAGCGTGCC CGGCCACGGCACGGCACGGCGCGGGAGCACAGGGGTCTGGCGCCCGTGCGCTGCCTCTCCCCCTTTGTATTTAGTTTTG TAGTTCTCAGCTTTTGTAATCCCAAGACTTTGAGGGTGAGCTGCCAGCATCCTCCTCTCCTGCTCCGGACTGCGGCTGC AGCGCGCACTCTGCCTTCCCGCGCCCGCCTGCACGCCGCCGGCACCCGCGGACTCCCACCTCCCCTTCTGCACTTTGGG ATTTCCCCTCGCCGCCTCCACGCCAAGTGCCCGTGCAGGGGCCCGGGAGCTGCGACGGATGCCGAGAGGAGCAGCGAGG AGCCCTGGGAGGAGGAGGATGCTTCGCCCAGGTGCCGAGACGCAGAGAACTGTGCAGGGAGGAAGCTGGCAAACCCGAT TTACTACTACTACTATTATTATTATTATAATTATTTTAATTTTTTTGTAACATTTTTATTTTTGTGAGTCGTGGCGGGA CCCCCCGCCCTGGGCACATGCCACAGCCTCCTTCCAGCACGCCGTCCTGCTGGCGCAGGCAGATCCGCCGCTGCCGTTT TAAACTTTTGTGTTCGTAGCTGTTGACTTTTGTTTTAGTCTCTTTATAAAAA
Citation	Mellott DO, and Burke RD. Divergent roles for Eph and Ephrin in Avian Cranial Neural Crest. BMC Developmental Biology 2008, 8:56.
Copyright	© 2008 Mellott and Burke; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI U72394.1 Authors said: The ephrin-B1 fragment amplified by primers ephrin-B1-F1 (5'-ATCGATGAGTGGGAAAGGGTTGGTC-3') and ephrin-B1-R1 (5-GTCGACGTTGTCTGCCTCCTTGCTG-3') was subcloned between the ClaI and SalI sites of pBluescript II SK +.