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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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GJA5
gap junction protein alpha 5

The human orthologue of this gene is associated
with the following human disease:

Atrial fibrillation, familial, 11

Gene

Official Symbol	GJA5
Official Full Name	gap junction protein alpha 5
CGNC ID	49840
also known as	gap junction alpha-5 protein\|connexin-42\|cx42\|gap junction protein, alpha 5, 40kDa (connexin 40)
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205504
Polypeptide	NP_990835
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.863

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	2702	ENSG00000265107	MIM:608583	MIM:121013
Mouse	14613	ENSMUSG00000057123	All phenotypic alleles (8):Targeted, knock-out(4) Targeted, other(4)	MGI:95716
Xenopus	100489536			490480
Zebrafish	404729, 570445	ENSDARG00000040065, ENSDARG00000069450	ZDB-GENE-040407-3, ZDB-GENE-050616-11	ZFIN:ZDB-GENE-040407-3, ZFIN:ZDB-GENE-050616-11

Gene Ontology

Molecular Function
Biological Process	cell communication
Cellular Component	connexon complex, integral component of membrane

Links to other databases

Entrez Gene	396502
Ensembl Gene	ENSGALG00000015487
KEGG	gga:396502

Entry 1

GEISHA Id	GJA5.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	5’-CACCCTGTCAACTGCTACGT-3’
Reverse cDNA Template Primer	5’-AATTAACCCTCACTAAAGGGGAACAGGAGGACGCTGAAA-3’	cDNA Template Length	632
Complete cDNA Template Probe	show CACCCTGTCAACTGCTACGTTTCCCGTCCCACAGAGAAGAACGTGTTCATCATCTTCATGCTTGCTGTGGCTGTGCTCT CCCTGTTCCTCAGCCTGGCTGAGTTATACCACCTGGGCTGGAAGAAAGCCAAAGAGAGGTGCTCCCGTGCTTACAAGCC CAGCCCTAGCACAGCTCCTCGAAGGCTGGAGTCCGCCCCACAGGTAGAAAGGGCCCAGATGTACACTCCCCCACCAGAT TTTAATCAGTGTTTGGCAAGTCCCAATGGGAAGTTCATCAGCCCCTTCAGCAACAAGATGGCCTCGCAGCAGAACACAG CCAACTTTGCCACTGAGAGGGTCCACAGCCAGGAGGATGCTGCCGGGGAAGGGCCCTTCATGAAGTCCAGCTACATGGA AAGCCCAGAGGTGGCCAGTGAATGTGCGGCACCCGCCCTCCCTGAGAGCTACTTCAATGAGAAACGCCGCTTCAGCAAG GCCAGCCGGGCCAGCAGCAAGGCGAGGTCAGACGATTTGTCGGTGTGATCTGTCTCCAGGAAAGAGGAGGAGTGGTCCC AGCGCTTAGTAAAACTTCTGCAAAGTGGACTGCAGACTCTTGCCACAGTCCTCTCTCCATTTCAGCGTCCTCCTGTTCC

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 10

stage 12

Aorta
Blood Vessels
Extraembryonic Blood Vessels
Unlabeled

13-18

stage 16

stage 18

Aorta
Blood Vessels
Extraembryonic Blood Vessels
Outflow Tract
Sinus Venosus
Ventricles

19-24

stage 19

stage 23

Aorta
Arteries
Atria
Blood Vessels
Extraembryonic Blood Vessels
Eye
Leg Mesenchyme
Myotome
Outflow Tract
Pharyngeal Arches and Clefts
Sinus Venosus
Ventricles
Wing Mesenchyme

stage 25

Atria
Blood Islands
Blood Vessels
Leg Mesenchyme
Myotome
Ventricles
Wing Mesenchyme

Entry 2

GEISHA Id	CX42.Berthoud.2004	Data Source	Publication
Complete cDNA Template Probe	show GGGCCCTTCATGAAGTCCAGCTACATGGAAAGCCCAGAGGTGGCCAGTGAATGTGCGGCACCCGCCCTCCCTGAGAGCT ACTTCAATGAGAAACGCCGCTTCAGCAAGGCCAGCCGGGCCAGCAGCAAGGCGAGGTCAGACGATTTGTCGGTGTGATC TGTCTCCAGGAAAGAGGAGGAGTGGTCCCAGCGCTTAGTAAAACTTCTGCAAAGTGGACTGCAGACTCTTGCCACAGTC CTCTCTCCATTTCAGCGTCCTCCTGTTCCTTTTCAGTGAACATCACTCTTGTAATTCAGACAGCACTTCTCACAGCTTA CAGCAGGGGCAGACCCTGGACAATACATACCTGGAGGTTGAAAAGAGGCACCCATCTCTAGATGTGAAGTTCTCAGCCC GTGGGACTCAAGGGGCCTGGGGACTCACCAACCTTAGACACAGGCTGGAACACTCCTGATGCTGCCTGGATGCCTCAGA TGTGAGGTAGCCGTCACTGCGACTCTCTGTTCAGCAGACTGACTGGACCTCAACCCTCATGTGTCTTCTTTGATGTCTG TTCAACATGTGCTAAAACAGGTGGGCAGTTATACTGAGCTGAAGCTTCTCTCACCTGCAGGGATCAGAAACACACTTTC AGAAAGACTTCCTCTTTTTCTGTGCTCTTTCTCTTTCTAGCCCCCTCCCCTTCTCCACTTAAGCCCAGATATTTTAATC CAGTTTACTTATACAATAAATCTTACTTGAATTTTTGACACTGTGCATAAAACTCTTCCCCGTGAACACGGATGCCCAT GGTCCCCCAAGCACAGCTGCCTTGTGTCTAACTTTGGAATCCCTTCCTCATTCCTAAGTCAGCGGCGGGCTGGCCCCAT GGCCTGGGATATCGCTCAGATGACTGGTTGATGAAGACAGGAAATGTTCCCAACATACTTATGTCATCAATTATAAGCA AGACACCCGCTAACAGAAATAGAGATTGTCCTGTGAGAACACATATACAACTACTAAAAAGGGAGGAATTATCACATGG AAACAAAGTGACTTTTCCCCCTTTTAAGCTCATCTTTCAAGCAACCTGAATGCTGCTGAAAGGGTGCTACCAGCTGCCA ACTTGGGAGGGAAACATCCTCTGTTTACATGCAGAACAGGTACAGCCTGTGTTTACTTGCTACCACCCTACCAAAATAC TTCAGCATCTTTTGCAAGGAGCAAGAGTCCTGCACATCACTGTCCAGGTCCTGCACATCACTGAGATCCTGGCTGCTCT CTCAAGCCTGCCAGCCACTGCAAGCAGCAATATTTGTCCTCTCAGCAAATCTCTGTTCTGCAAACAGGTCGTCTTCATC TTTACTGCTAGTGTACACAGAACAAGAAACCACACGCAGCCACCTTTTTTCACCACTGTAACCTCCCAGAGCAGCTGTG GAGCCCAGCACTGCACAGTGAACCTGGTGGGTGAGGTCTGCAGGCAGCTTTCACCCTGGACATGTTTTCTCACTGCAGA GCTGACATACTGAGACCAACCAAAGCCCCACTGCTGCCCGTTGTTTACCCTGCACTGCCCTTTCAGACACGGGCTACAG CTTCCCAGTGTTGGACTGTGTGAGCGAATGGCCTGGGACCAGATTGTCAGGGG
Citation	Berthoud VM, Singh R, Minogue PJ, Ragsdale CW, Beyer EC. Highly restricted pattern of connexin36 expression in chick somite development. Anat Embryol (Berl). 2004 Nov;209(1):11-8.
Copyright	© Springer-Verlag 2004
Comments	Authors indicated the probe could be found in another publication (See text). The indicated probe sequence is included here.

Stage

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Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Aorta
Blood Vessels
Myotome

double label

Entry 3

GEISHA Id	GJA5.Buschmann.2010	Data Source	Publication
Complete cDNA Template Probe	show GGCCGAACGGGACGGAGAGGTGGGGTGCAGTGAGACAGAGGGGCTGTGCTGAAGCAAACACTTCAGTCAGCCACCCGCT CCTCCCTCCACCTGTGCAGGCTCTGCAAAAAGAGAGGCCGTCTCCAATTCTGCCTTCAGGACTTCTACACACTCCTGGT GAGCAGAAATGGGAGATTGGAGTTTCCTGGGAGAGTTCCTTGAGGAGGTCCACAAGCACTCCACAGTGGTGGGGAAGGT CTGGTTGACTGTGCTGTTCATCTTCCGGATGCTGGTGCTTGGTACAGCAGCTGGTCCTCTTTGGGGGGATGAGCAGTCC GATTTCATGTGTGACACCCAGCAGCCTGGCTGTGAAAACGTCTGCTACGACAAGGCCTTCCCCATTTCCCACGTCCGCT TTTGGGTCCTTCAGATCATCTTTGTCTCCACTCCATCCCTGGTGTATATGGGCCACGCAATGCATACGGTGCGCATGGA GGAGAAGAGAAAGATGAAGGAGGCAGAAAGAGAGGCCCAGGAGATGAAAAACAGTGGTGACACGTACTATCAGCAGAAG TGCCCCGTGGCAGAGAAGACTGAGTTGTCTTGCTGGGACGAATCAGGAGGCAAAATTATACTCAGAGGCAGTCTGCTAA ACACCTATGTCTACAGTATTTTGATTCGCACTGCCATGGAAATCGCCTTCATTGTGGGGCAATACATCTTGTATGGGAT CTTCCTGGAGACCCTGTATATCTGCCAGCGGGCACCTTGCCCCCACCCTGTCAACTGCTACGTTTCCCGTCCCACAGAG AAGAACGTGTTCATCATCTTCATGCTTGCTGTGGCTGTGCTCTCCCTGTTCCTCAGCCTGGCTGAGTTATACCACCTGG GCTGGAAGAAAGCCAAAGAGAGGTGCTCCCGTGCTTACAAGCCCAGCCCTAGCACAGCTCCTCGAAGGCTGGAGTCCGC CCCACAGGTAGAAAGGGCCCAGATGTACACTCCCCCACCAGATTTTAATCAGTGTTTGGCAAGTCCCAATGGGAAGTTC ATCAGCCCCTTCAGCAACAAGATGGCCTCGCAGCAGAACACAGCCAACTTTGCCACTGAGAGGGTCCACAGCCAGGAGG ATGCTGCCGGGGAAGGGCCCTTCATGAAGTCCAGCTACATGGAAAGCCCAGAGGTGGCCAGTGAATGTGCGGCACCCGC CCTCCCTGAGAGCTACTTCAATGAGAAACGCCGCTTCAGCAAGGCCAGCCGGGCCAGCAGCAAGGCGAGGTCAGACGAT TTGTCGGTGTGATCTGTCTCCAGGAAAGAGGAGGAGTGGTCCCAGCGCTTAGTAAAACTTCTGCAAAGTGGACTGCAGA CTCTTGCCACAGTCCTCTCTCCATTTCAGCGTCCTCCTGTTCCTTTTCAGTGAACATCACTCTTGTAATTCAGACAGCA CTTCTCACAGCTTACAGCAGGGGCAGACCCTGGACAATACATACCTGGAGGTTGAAAAGAGGCACCCATCTCTAGATGT GAAGTTCTCAGCCCGTGGGACTCAAGGGGCCTGGGGACTCACCAACCTTAGACACAGGCTGGAACACTCCTGATGCTGC CTGGATGCCTCAGATGTGAGGTAGCCGTCACTGCGACTCTCTGTTCAGCAGACTGACTGGACCTCAACCCTCATGTGTC TTCTTTGATGTCTGTTCAACATGTGCTAAAACAGGTGGGCAGTTATACTGAGCTGAAGCTTCTCTCACCTGCAGGGATC AGAAACACACTTTCAGAAAGACTTCCTCTTTTTCTGTGCTCTTTCTCTTTCTAGCCCCCTCCCCTTCTCCACTTAAGCC CAGATATTTTAATCCAGTTTACTTATACAATAAATCTTACTTGAATTTTTGACACTGTGCATAAAACTCTTCCCCGTGA ACACGGATGCCCATGGTCCCCCAAGCACAGCTGCCTTGTGTCTAACTTTGGAATCCCTTCCTCATTCCTAAGTCAGCGG CGGGCTGGCCCCATGGCCTGGGATATCGCTCAGATGACTGGTTGATGAAGACAGGAAATGTTCCCAACATACTTATGTC ATCAATTATAAGCAAGACACCCGCTAACAGAAATAGAGATTGTCCTGTGAGAACACATATACAACTACTAAAAAGGGAG GAATTATCACATGGAAACAAAGTGACTTTTCCCCCTTTTAAGCTCATCTTTCAAGCAACCTGAATGCTGCTGAAAGGGT GCTACCAGCTGCCAACTTGGGAGGGAAACATCCTCTGTTTACATGCAGAACAGGTACAGCCTGTGTTTACTTGCTACCA CCCTACCAAAATACTTCAGCATCTTTTGCAAGGAGCAAGAGTCCTGCACATCACTGTCCAGGTCCTGCACATCACTGAG ATCCTGGCTGCTCTCTCAAGCCTGCCAGCCACTGCAAGCAGCAATATTTGTCCTCTCAGCAAATCTCTGTTCTGCAAAC AGGTCGTCTTCATCTTTACTGCTAGTGTACACAGAACAAGAAACCACACGCAGCCACCTTTTTTCACCACTGTAACCTC CCAGAGCAGCTGTGGAGCCCAGCACTGCACAGTGAACCTGGTGGGTGAGGTCTGCAGGCAGCTTTCACCCTGGACATGT TTTCTCACTGCAGAGCTGACATACTGAGACCAACCAAAGCCCCACTGCTGCCCGTTGTTTACCCTGCACTGCCCTTTCA GACACGGGCTACAGCTTCCCAGTGTTGGACTGTGTGAGCGAATGGCCTGGGACCAGATTGTCAGGGG
Citation	Buschmann I, Pries A, Styp-Rekowska B, Hillmeister P, Loufrani L, Henrion D, Shi Y, Duelsner A, Hoefer I, Gatzke N, Wang H, Lehmann K, Ulm L, Ritter Z, Hauff P, Hlushchuk R, Djonov V, van Veen T, le Noble F. Pulsatile shear and Gja5 modulate arterial identity and remodeling events during flow-driven arteriogenesis. Development. 2010 Jul;137(13):2187-96.
Copyright	Published by the Company of Biologists Ltd. Copyright held by the authors.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205504.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

13-18

stage 13

stage 14

stage 18

Aorta
Arteries
Blood Vessels
Central Nervous System
Heart

Entry 4

GEISHA Id	CX40.Chau.2006	Data Source	Publication
Complete cDNA Template Probe	show GGCCGAACGGGACGGAGAGGTGGGGTGCAGTGAGACAGAGGGGCTGTGCTGAAGCAAACACTTCAGTCAGCCACCCGCT CCTCCCTCCACCTGTGCAGGCTCTGCAAAAAGAGAGGCCGTCTCCAATTCTGCCTTCAGGACTTCTACACACTCCTGGT GAGCAGAAATGGGAGATTGGAGTTTCCTGGGAGAGTTCCTTGAGGAGGTCCACAAGCACTCCACAGTGGTGGGGAAGGT CTGGTTGACTGTGCTGTTCATCTTCCGGATGCTGGTGCTTGGTACAGCAGCTGGTCCTCTTTGGGGGGATGAGCAGTCC GATTTCATGTGTGACACCCAGCAGCCTGGCTGTGAAAACGTCTGCTACGACAAGGCCTTCCCCATTTCCCACGTCCGCT TTTGGGTCCTTCAGATCATCTTTGTCTCCACTCCATCCCTGGTGTATATGGGCCACGCAATGCATACGGTGCGCATGGA GGAGAAGAGAAAGATGAAGGAGGCAGAAAGAGAGGCCCAGGAGATGAAAAACAGTGGTGACACGTACTATCAGCAGAAG TGCCCCGTGGCAGAGAAGACTGAGTTGTCTTGCTGGGACGAATCAGGAGGCAAAATTATACTCAGAGGCAGTCTGCTAA ACACCTATGTCTACAGTATTTTGATTCGCACTGCCATGGAAATCGCCTTCATTGTGGGGCAATACATCTTGTATGGGAT CTTCCTGGAGACCCTGTATATCTGCCAGCGGGCACCTTGCCCCCACCCTGTCAACTGCTACGTTTCCCGTCCCACAGAG AAGAACGTGTTCATCATCTTCATGCTTGCTGTGGCTGTGCTCTCCCTGTTCCTCAGCCTGGCTGAGTTATACCACCTGG GCTGGAAGAAAGCCAAAGAGAGGTGCTCCCGTGCTTACAAGCCCAGCCCTAGCACAGCTCCTCGAAGGCTGGAGTCCGC CCCACAGGTAGAAAGGGCCCAGATGTACACTCCCCCACCAGATTTTAATCAGTGTTTGGCAAGTCCCAATGGGAAGTTC ATCAGCCCCTTCAGCAACAAGATGGCCTCGCAGCAGAACACAGCCAACTTTGCCACTGAGAGGGTCCACAGCCAGGAGG ATGCTGCCGGGGAAGGGCCCTTCATGAAGTCCAGCTACATGGAAAGCCCAGAGGTGGCCAGTGAATGTGCGGCACCCGC CCTCCCTGAGAGCTACTTCAATGAGAAACGCCGCTTCAGCAAGGCCAGCCGGGCCAGCAGCAAGGCGAGGTCAGACGAT TTGTCGGTGTGATCTGTCTCCAGGAAAGAGGAGGAGTGGTCCCAGCGCTTAGTAAAACTTCTGCAAAGTGGACTGCAGA CTCTTGCCACAGTCCTCTCTCCATTTCAGCGTCCTCCTGTTCCTTTTCAGTGAACATCACTCTTGTAATTCAGACAGCA CTTCTCACAGCTTACAGCAGGGGCAGACCCTGGACAATACATACCTGGAGGTTGAAAAGAGGCACCCATCTCTAGATGT GAAGTTCTCAGCCCGTGGGACTCAAGGGGCCTGGGGACTCACCAACCTTAGACACAGGCTGGAACACTCCTGATGCTGC CTGGATGCCTCAGATGTGAGGTAGCCGTCACTGCGACTCTCTGTTCAGCAGACTGACTGGACCTCAACCCTCATGTGTC TTCTTTGATGTCTGTTCAACATGTGCTAAAACAGGTGGGCAGTTATACTGAGCTGAAGCTTCTCTCACCTGCAGGGATC AGAAACACACTTTCAGAAAGACTTCCTCTTTTTCTGTGCTCTTTCTCTTTCTAGCCCCCTCCCCTTCTCCACTTAAGCC CAGATATTTTAATCCAGTTTACTTATACAATAAATCTTACTTGAATTTTTGACACTGTGCATAAAACTCTTCCCCGTGA ACACGGATGCCCATGGTCCCCCAAGCACAGCTGCCTTGTGTCTAACTTTGGAATCCCTTCCTCATTCCTAAGTCAGCGG CGGGCTGGCCCCATGGCCTGGGATATCGCTCAGATGACTGGTTGATGAAGACAGGAAATGTTCCCAACATACTTATGTC ATCAATTATAAGCAAGACACCCGCTAACAGAAATAGAGATTGTCCTGTGAGAACACATATACAACTACTAAAAAGGGAG GAATTATCACATGGAAACAAAGTGACTTTTCCCCCTTTTAAGCTCATCTTTCAAGCAACCTGAATGCTGCTGAAAGGGT GCTACCAGCTGCCAACTTGGGAGGGAAACATCCTCTGTTTACATGCAGAACAGGTACAGCCTGTGTTTACTTGCTACCA CCCTACCAAAATACTTCAGCATCTTTTGCAAGGAGCAAGAGTCCTGCACATCACTGTCCAGGTCCTGCACATCACTGAG ATCCTGGCTGCTCTCTCAAGCCTGCCAGCCACTGCAAGCAGCAATATTTGTCCTCTCAGCAAATCTCTGTTCTGCAAAC AGGTCGTCTTCATCTTTACTGCTAGTGTACACAGAACAAGAAACCACACGCAGCCACCTTTTTTCACCACTGTAACCTC CCAGAGCAGCTGTGGAGCCCAGCACTGCACAGTGAACCTGGTGGGTGAGGTCTGCAGGCAGCTTTCACCCTGGACATGT TTTCTCACTGCAGAGCTGACATACTGAGACCAACCAAAGCCCCACTGCTGCCCGTTGTTTACCCTGCACTGCCCTTTCA GACACGGGCTACAGCTTCCCAGTGTTGGACTGTGTGAGCGAATGGCCTGGGACCAGATTGTCAGGGG
Citation	Mary D.L. Chau, Richard Tuft, Kevin Fogarty, Zheng-Zheng Bao. Notch signaling plays a key role in cardiac cell differentiation. Mech Dev. 2006 August; 123(8): 626640. Published online 2006 July 14.
Copyright	Copyright © 2006 Elsevier Ireland Ltd. All rights reserved
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # NM_205504.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

25-29

stage 29

Endocardium
Myocardium
Ventricles

Entry 5

GEISHA Id	GJA5.Anderson.2021	Data Source	Direct Submission
Complete cDNA Template Probe	show GCAAGTCCCAATGGGAAGTTCATCAGCCCCTTCAGCAACAAGATGGCCTCGCAGCAGAACACAGCCAACTTTGCCACTG AGAGGGTCCACAGCCAGGAGGATGCTGCCGGGGAAGGGCCCTTCATGAAGTCCAGCTACATGGAAAGCCCAGAGGTGGC CAGTGAATGTGCGGCACCCGCCCTCCCTGAGAGCTACTTCAATGAGAAACGCCGCTTCAGCAAGGCCAGCCGGGCCAGC AGCAAGGCGAGGTCAGACGATTTGTCGGTGTGATCTGTCTCCAGGAAAGAGGAGGAGTGGTCCCAGCGCTTAGTAAAAC TTCTGCAAAGTGGACTGCAGACTCTTGCCACAGTCCTCTCTCCATTTCAGCGTCCTCCTGTTCCTTTTCAGTGAACATC ACTCTTGTAATTCAGACAGCACTTCTCACAGCTTACAGCAGGGGCAGACCCTGGACAATACATACCTGGAGGTTGAAAA GAGGCACCCATCTCTAGATGTGAAGTTCTCAGCCCGTGGGACTCAAGGGGCCTGGGGACTCACCAACCTTAGACACAGG CTGGAACACTCCTGATGCTGCCTGGATGCCTCAGATGTGAGGTAGCCGTCACTGCGACTCTCTGTTCAGCAGACTGACT GGACCTCAACCCTCATGTGTCTTCTTTGATGTCTGTTCAACATGTGCTAAAACAGGTGGGCAGTTATACTGAGCTGAAG CTTCTCTCACCTGCAGGGATCAGAAACACACTTTCAGAAAGACTTCCTCTTTTTCTGTGCTCTTTCTCTTTCTAGCTCC CTCC
Comments	Images and probe sequence provided by Claire Anderson. The probe was synthesized from cDNA clone ChEST304a4. Related paper: Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 7:12656. doi: 10.1038/ncomms12656.