Geisha

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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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WNT8A
Wnt family member 8A

Gene

Official Symbol	WNT8A
Official Full Name	Wnt family member 8A
CGNC ID	4563
also known as	CWNT-8C, Wnt8c, protein Wnt-8c\|CWnt-8\|Wnt-8C\|wingless-type MMTV integration site family, member 8A, CWnt-8, Wnt8a, Wnt-8c protein precursor
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_205531
Polypeptide	NP_990862
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.886

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	7478	ENSG00000061492		MIM:606360
Mouse	20890	ENSMUSG00000012282	All phenotypic alleles (1):Targeted, knock-out(1)	MGI:107924
Xenopus	549962, 397970			493705
Zebrafish	30122		ZDB-GENE-980526-332	ZFIN:ZDB-GENE-980526-332

Gene Ontology

Molecular Function	receptor binding
Biological Process	embryonic hindlimb morphogenesis, more...
Cellular Component	proteinaceous extracellular matrix

Links to other databases

Entrez Gene	396543
Ensembl Gene	ENSGALG00000006084
KEGG	gga:396543

Entry 1

GEISHA Id	WNT8A.UApcr	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show CATCACAAACAAAATCGGGAACTTGTGTAAAGCCCGATGAAGGAGCTCAAGTCCATGGGGTTCAGGCAGTTGCAGATCC AACATGCTGGCTCCAGCTGCGACTTTGGGAGATTGGCAACTACCTGAAGATGAAATACGACAGGCATAAGCTGGAGATG GACAAAGCGGATGAGACTGGACAGCCGACAGCCGGCGCCTTGAACCTTCCCGTTCACTCAGAGCTGTCTTTTTGGAGGA CTCCCATACTGCACGAGAACGGCTGGCCACGCACAGGGGGAGTGCCTAAGGCAAGAATCTTCATGGGAGCGGAGGACTG CCGCGGCTTCAGGATGCGGCCTCATGGAGAGAGAGGACAGAGTGGTCACACTGCAATAGTTCACTGGTGTGCACGGTGC GGTGACTCCGCTGGTGGCCAGCACTTCTTGCCCGCCGGATGCGCCGTTGCCAACCACACAAGAACAAAGGCCACAAGAT AGGGAGGCTGGACATGCCTGCGGCGACTGTGGTTTTTCTTTGCGGCCCTACCTGCTAGGTTGAAGACCTTGCCCGAAGT CACTTTATATCAGACAGAGGAAGAATTTCATTTTCATGGGTTCACATTGCACCACGCGCCAGTCAATGTTACATGAGTG GACGCCCATCCGGGCTGGTCTGTTGGCTGTTTTGCCCCCAACATCTGATTGGAACTGAGGATTTTTTG

Stage

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Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 6

Early Mesoderm
Hensen's Node
Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak

7-12

stage 8

stage 10

stage 11

Hensen's Node
Lateral Plate Mesoderm
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
Tail

13-18

stage 14

stage 15

Tail

19-24

stage 20

stage 24

Tail

Entry 2

GEISHA Id	Wnt8c.Mikawa.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Mikawa, T, Poh, A, Kelly, K, Ishii, Y, Reese, D. Induction and Patterning of the Primitive Streak, an Organizing Center of Gastrulation in the Amniote. Dev Dyn. 2004 Mar;229(3):422-32.
Copyright	Copyright © 2004 Wiley-Liss, Inc.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Area Opaca
Primitive Streak

Chordin (red)
Posterior marginal zone
Wnt8c (bluish purple)

1-3

stage 2

stage 3

Area Opaca
Primitive Streak

Chordin (red)
Posterior marginal zone
Wnt8c (bluish purple)

4-6

stage 4

stage 6

Primitive Streak

Chordin (red)
Wnt8c (bluish purple)

Entry 3

GEISHA Id	Wnt8c.Chapman.2004	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Chapman, S, Brown, R, Lees, L, Schoenwolf, G, Lumsden, A. Expression Analysis of Chick Wnt and Frizzled Genes and Selected Inhibitors in Early Chick Patterning. Dev Dyn. 2004 Mar;229(3):668-76.
Copyright	Wiley-Liss, Inc. 2004

Stage

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Location (click to highlight)

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4-5

stage 4

stage 5

Primitive Streak

Entry 4

GEISHA Id	Wnt8c.Ehrman.2001	Data Source	Publication
Complete cDNA Template Probe	show GCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAG GAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAG CTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTG CTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCA GCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGG CGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCG TCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCT ACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAG GAGAGG
Citation	Ehrman LA, Yutzey KE. Anterior expression of the caudal homologue cCdx-B activates a posterior genetic program in avian embryos. Dev Dyn. 2001 Aug;221(4):412-21.
Copyright	2001 Wiley-Liss, Inc.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 5

Mesoderm, early
Primitive Streak

7-8

stage 7

stage 8

Mesoderm, early
Paraxial Mesoderm
Primitive Streak
Somites

Entry 5

GEISHA Id	Wnt8b.Garcia-Calero.2006	Data Source	Publication
Complete cDNA Template Probe	show ATGGGGCGGGGCAGGGCAGGCAGACAGGACCCCAATAGGTGTAGGCAGGAGAACGTGCTGAGGGACCAGAGGTCAGTGA ATAATTTCCTGATGACGGGCCCCAAGGCCTATCTCATCTACTCCAGCAGTGTGGCTGCTGGGGCACAGAGTGGCATTGA GGAGTGCAAGTTCCAGTTTGCCTGGGATCGCTGGAACTGCCCCGAGCGGGCACTGCAGCTCTCCAGCCATGGCGGGCTG CGCAGTGCAAACCGAGAAACTGCCTTTGTCCATGCCATCAGCTCTGCAGGTGTCATGTACACGCTGACCCGGAACTGCA GCCTGGGAGATTTTGACAACTGTGGCTGTGATGACTCCCGCAATGGGCAGCTTGGTGGGCAAGGCTGGCTGTGGGGAGG CTGCAGCGATAATGTGGGCTTTGGGGAAGCCATCTCCAAGCAGTTTGTGGATGCCCTGGAGACTGGACAAGATGCCAGA GCTGCTATGAACCTGCACAACAACGAGGCAGGAAGAAAGGCTGTCAAAGGGACCATGAAGCGGACTTGCAAATGCCACG GTGTTTCAGGAAGTTGTACCACACAGACCTGCTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCACTTACCTCAAGGA GAGGTACCACAAGGCCTTGAAGGTGGACTTGCTGCAGGGGGTGGGGAACAGCGCTGCCAGCCGGGGGGCCATTGCCGAG ACCTTCAGCTCCATCTCCAAGAAGGAGCTGGTCCATTTAGAAGACTCTCCCGACTACTGCCTGGAGAACAAGACGCTGG GACTGCTGGGCACGGAGGGCCGCGAGTGTCTGAAGAGAGGCAAGGCGCTCAGCAAGTGGGAGAAGCGGAGCTGCCGGCG GCTGTGTGGGGACTGTGGGCTGGCGGTGGAGGAGAGACGTGCCGAGATGGTGTCCAGCTGCAACTGCAAGTTCCATTGG TGCTGCGCCGTGCGGTGTGAGCAGTGCCGCAAACGGGTCACCAAGTACTTCTGTGTCCGCAAAGAGAAGCGGGAGCAGA GCGGGGGTGGCGGGGCCAGCCGCAAGCTCAAGAGGAAGCTCTAA
Citation	García-Calero E, Garda AL, Marín F, Puelles L. Expression of Lrrn1 marks the prospective site of the zona limitans thalami in the early embryonic chicken diencephalon. Gene Expr Patterns. 2006 Oct;6(8):879-85. Epub 2006 Feb 28.
Copyright	Reprinted with permission from Elsevier. Copyright © 2006 Elsevier B.V. All rights reserved.
Comments	According to the authors, "Digoxigenin-labeled RNA probes were prepared from plasmids kindly provided by ... A. McMahon(Wnt8b)." As information provided was insufficient to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (XM_426508).

Stage

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Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Forebrain
Telencephalon

Supramammillary Band
Zona Limitans Thalami

Entry 6

GEISHA Id	Wnt8c.Ladher.2000	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Ladher RK, Church VL, Allen S, Robson L, Abdelfattah A, Brown NA, Hattersley G, Rosen V, Luyten FP, Dale L, Francis-West PH. Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development. Dev Biol. 2000 Feb 15;218(2):183-98.
Copyright	Reprinted with permission from Elsevier. Copyright © 2000 Elsevier B.V. All rights reserved.
Comments	According to the authors, "... Wnt-8c (Hume and Dodd, 1993) were used as previously described." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (AB193181).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-12

stage 7

stage 12

Early Mesoderm
Hensen's Node
Neural Plate/Tube
Primitive Streak
Rhombomeres

Figure 3 compares the expression of Sfrp-2 and Frzb in the early developing embryo. Figure cover stages HH7, HH9 and HH12.
Posterior Neural Plate
Presumptive Mesoderm
Presumptive Rhombomere 4

Entry 7

GEISHA Id	Wnt8c.Baranski.2000	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Baranski M, Berdougo E, Sandler JS, Darnell DK, Burrus LW. The dynamic expression pattern of frzb-1 suggests multiple roles in chick development. Dev Biol. 2000 Jan 1;217(1):25-41.
Copyright	Copyright © 2000 Academic Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205531).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Primitive Streak

FIG. 2. Expression of cfrzb-1, Wnt-3a, Wnt-5a, and Wnt-8c in gastrula-stage chick embryos.

Entry 8

GEISHA Id	Wnt8c.Chapman.2008	Data Source	Direct Submission
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Comments	Insufficient information provided to verify exact sequence used to synthesize probe. The sequence below was obtained from NCBI (acc # AB193181). Images Provided by Susan Chapman.

Stage

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Location (click to highlight)

Comments (click to highlight)

1-3

stage 1

stage 2

stage 3

Primitive Streak
Unlabeled

4-6

stage 4

stage 5

stage 6

Early Endoderm
Early Mesoderm
Epiblast
Neural Plate/Tube
Primitive Streak

7-11

stage 8

stage 9

stage 10

stage 11

Central Nervous System
Diencephalon
Forebrain
Head Mesenchyme
Hindbrain
Lateral Plate Mesoderm
Midbrain
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak
Spinal Cord

Entry 9

GEISHA Id	Wnt8b.Vieira.2005	Data Source	Publication
Complete cDNA Template Probe	show ATGGGGCGGGGCAGGGCAGGCAGACAGGACCCCAATAGGTGTAGGCAGGAGAACGTGCTGAGGGACCAGAGGTCAGTGA ATAATTTCCTGATGACGGGCCCCAAGGCCTATCTCATCTACTCCAGCAGTGTGGCTGCTGGGGCACAGAGTGGCATTGA GGAGTGCAAGTTCCAGTTTGCCTGGGATCGCTGGAACTGCCCCGAGCGGGCACTGCAGCTCTCCAGCCATGGCGGGCTG CGCAGTGCAAACCGAGAAACTGCCTTTGTCCATGCCATCAGCTCTGCAGGTGTCATGTACACGCTGACCCGGAACTGCA GCCTGGGAGATTTTGACAACTGTGGCTGTGATGACTCCCGCAATGGGCAGCTTGGTGGGCAAGGCTGGCTGTGGGGAGG CTGCAGCGATAATGTGGGCTTTGGGGAAGCCATCTCCAAGCAGTTTGTGGATGCCCTGGAGACTGGACAAGATGCCAGA GCTGCTATGAACCTGCACAACAACGAGGCAGGAAGAAAGGCTGTCAAAGGGACCATGAAGCGGACTTGCAAATGCCACG GTGTTTCAGGAAGTTGTACCACACAGACCTGCTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCACTTACCTCAAGGA GAGGTACCACAAGGCCTTGAAGGTGGACTTGCTGCAGGGGGTGGGGAACAGCGCTGCCAGCCGGGGGGCCATTGCCGAG ACCTTCAGCTCCATCTCCAAGAAGGAGCTGGTCCATTTAGAAGACTCTCCCGACTACTGCCTGGAGAACAAGACGCTGG GACTGCTGGGCACGGAGGGCCGCGAGTGTCTGAAGAGAGGCAAGGCGCTCAGCAAGTGGGAGAAGCGGAGCTGCCGGCG GCTGTGTGGGGACTGTGGGCTGGCGGTGGAGGAGAGACGTGCCGAGATGGTGTCCAGCTGCAACTGCAAGTTCCATTGG TGCTGCGCCGTGCGGTGTGAGCAGTGCCGCAAACGGGTCACCAAGTACTTCTGTGTCCGCAAAGAGAAGCGGGAGCAGA GCGGGGGTGGCGGGGCCAGCCGCAAGCTCAAGAGGAAGCTCTAA
Citation	Vieira C, Garda AL, Shimamura K, Martinez S. Thalamic development induced by Shh in the chick embryo. Dev Biol. 2005 Aug 15;284(2):351-63.
Copyright	Copyright © 2005 Elsevier Inc. All rights reserved.
Comments	According to the authors, "Digoxigenin and fluorescein-labeled RNA probes were prepared from plasmids kindly provided by A. McMahon." As information provided was insufficient to verify exact sequence used to synthesize probe,the sequence below was obtained from NCBI (XM_426508).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 25

Forebrain
Midbrain

zona limitans intrathalamica.

Entry 10

GEISHA Id	Wnt8c.Skromne.2001	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Skromne I, Stern CD. Interactions between Wnt and Vg1 signalling pathways initiate primitive streak formation in the chick embryo. Development. 2001 Aug;128(15):2915-27
Copyright	Printed in Great Britain © The Company of Biologists Limited 2001
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205531).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XII

Area Opaca

Marginal Zone

Entry 11

GEISHA Id	Wnt8c.Joubin.1999	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Joubin K, Stern CD. Molecular interactions continuously define the organizer during the cell movements of gastrulation. Cell. 1999 Sep 3;98(5):559-71.
Copyright	Copyright © 1999 Cell Press. All rights reserved.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (NM_205531).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Primitive Streak

Entry 12

GEISHA Id	Wnt8b.Theodosiou.2003	Data Source	Publication
Complete cDNA Template Probe	show ATGGGGCGGGGCAGGGCAGGCAGACAGGACCCCAATAGGTGTAGGCAGGAGAACGTGCTGAGGGACCAGAGGTCAGTGA ATAATTTCCTGATGACGGGCCCCAAGGCCTATCTCATCTACTCCAGCAGTGTGGCTGCTGGGGCACAGAGTGGCATTGA GGAGTGCAAGTTCCAGTTTGCCTGGGATCGCTGGAACTGCCCCGAGCGGGCACTGCAGCTCTCCAGCCATGGCGGGCTG CGCAGTGCAAACCGAGAAACTGCCTTTGTCCATGCCATCAGCTCTGCAGGTGTCATGTACACGCTGACCCGGAACTGCA GCCTGGGAGATTTTGACAACTGTGGCTGTGATGACTCCCGCAATGGGCAGCTTGGTGGGCAAGGCTGGCTGTGGGGAGG CTGCAGCGATAATGTGGGCTTTGGGGAAGCCATCTCCAAGCAGTTTGTGGATGCCCTGGAGACTGGACAAGATGCCAGA GCTGCTATGAACCTGCACAACAACGAGGCAGGAAGAAAGGCTGTCAAAGGGACCATGAAGCGGACTTGCAAATGCCACG GTGTTTCAGGAAGTTGTACCACACAGACCTGCTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCACTTACCTCAAGGA GAGGTACCACAAGGCCTTGAAGGTGGACTTGCTGCAGGGGGTGGGGAACAGCGCTGCCAGCCGGGGGGCCATTGCCGAG ACCTTCAGCTCCATCTCCAAGAAGGAGCTGGTCCATTTAGAAGACTCTCCCGACTACTGCCTGGAGAACAAGACGCTGG GACTGCTGGGCACGGAGGGCCGCGAGTGTCTGAAGAGAGGCAAGGCGCTCAGCAAGTGGGAGAAGCGGAGCTGCCGGCG GCTGTGTGGGGACTGTGGGCTGGCGGTGGAGGAGAGACGTGCCGAGATGGTGTCCAGCTGCAACTGCAAGTTCCATTGG TGCTGCGCCGTGCGGTGTGAGCAGTGCCGCAAACGGGTCACCAAGTACTTCTGTGTCCGCAAAGAGAAGCGGGAGCAGA GCGGGGGTGGCGGGGCCAGCCGCAAGCTCAAGAGGAAGCTCTAA
Citation	Theodosiou NA, Tabin CJ. Wnt signaling during development of the gastrointestinal tract. Dev Biol. 2003 Jul 15;259(2):258-71.
Copyright	Copyright © 2003 Elsevier Science (USA). All rights reserved.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc # XM_426508.2).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-24

stage 24

Duodenum
Foregut
Gizzard
Gut
Stomach

31-32

stage 32

Duodenum
Foregut
Midgut
Stomach

Entry 13

GEISHA Id	Wnt8c.Garcia-Castro.2002	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	García-Castro MI, Marcelle C, Bronner-Fraser M. Ectodermal Wnt function as a neural crest inducer. Science. 2002 Aug 2;297(5582):848-51.
Copyright	Copyright © 2002, The American Association for the Advancement of Science
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc. #NM_205531.1)

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Neural Plate/Tube
Primitive Streak
Surface Ectoderm

Entry 14

GEISHA Id	WNT8C.Olivera-Martinez.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Olivera-Martinez I, Storey KG., Wnt signals provide a timing mechanism for the FGF-retinoid differentiation switch during vertebrate body axis extension. Development. 2007 Jun;134(11):2125-35.
Copyright	Published by The Company of Biologists 2007. This material is protected by a copyright retained by the authors. Permission granted by Dr. Storey.
Comments	Authors indicated they had the clone associated with NCBI Acc# AF181680, and we inferred this was used as their probe. Its sequence is shown here.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Neural Plate/Tube

Entry 15

GEISHA Id	WNT8A.Hardy.2011	Data Source	Publication
Complete cDNA Template Probe	show CATCACAAACAAAATCGGGAACTTGTGTAAAGCCCGATGAAGGAGCTCAAGTCCATGGGGTTCAGGCAGTTGCAGATCC AACATGCTGGCTCCAGCTGCGACTTTGGGAGATTGGCAACTACCTGAAGATGAAATACGACAGGCATAAGCTGGAGATG GACAAAGCGGATGAGACTGGACAGCCGACAGCCGGCGCCTTGAACCTTCCCGTTCACTCAGAGCTGTCTTTTTGGAGGA CTCCCATACTGCACGAGAACGGCTGGCCACGCACAGGGGGAGTGCCTAAGGCAAGAATCTTCATGGGAGCGGAGGACTG CCGCGGCTTCAGGATGCGGCCTCATGGAGAGAGAGGACAGAGTGGTCACACTGCAATAGTTCACTGGTGTGCACGGTGC GGTGACTCCGCTGGTGGCCAGCACTTCTTGCCCGCCGGATGCGCCGTTGCCAACCACACAAGAACAAAGGCCACAAGAT AGGGAGGCTGGACATGCCTGCGGCGACTGTGGTTTTTCTTTGCGGCCCTACCTGCTAGGTTGAAGACCTTGCCCGAAGT CACTTTATATCAGACAGAGGAAGAATTTCATTTTCATGGGTTCACATTGCACCACGCGCCAGTCAATGTTACATGAGTG GACGCCCATCCGGGCTGGTCTGTTGGCTGTTTTGCCCCCAACATCTGATTGGAACTGAGGATTTTTTG
Citation	Hardy KM, Yatskievych TA, Konieczka J, Bobbs AS, Antin PB. FGF signalling through RAS/MAPK and PI3K pathways regulates cell movement and gene expression in the chicken primitive streak without affecting E-cadherin expression. BMC Dev Biol. 2011 Mar 21;11:20.
Copyright	© 2011 Hardy et al; licensee BioMed Central Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # NM_205531).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Early Mesoderm
Epiblast
Primitive Streak

Entry 16

GEISHA Id	WNT8C.Chapman.2007	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Chapman SC, Matsumoto K, Cai Q, Schoenwolf GC. Specification of germ layer identity in the chick gastrula. BMC Dev Biol. 2007 Jul 30;7:91.
Copyright	© 2007 Chapman et al; licensee BioMed Central Ltd.
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # AB193181.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

1-3

stage 3

Primitive Streak

Stage 3d chicken embryo

Entry 17

GEISHA Id	WNT8.Alev.2010	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Alev C, Wu Y, Kasukawa T, Jakt LM, Ueda HR, Sheng G. Transcriptomic landscape of the primitive streak. Development. 2010 Sep 1;137(17):2863-74. Epub 2010 Jul 28.
Copyright	Published by The Company of Biologists 2010. This material is protected by a copyright retained by the authors. Permission granted by Dr. G Sheng.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #NM_205531.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

stage 4

Early Mesoderm
Primitive Streak

Entry 18

GEISHA Id	WNT8B.Sienknecht.2009	Data Source	Publication
Complete cDNA Template Probe	show ATGGGGCGGGGCAGGGCAGGCAGACAGGACCCCAATAGGTGTAGGCAGGAGAACGTGCTGAGGGACCAGAGGTCAGTGA ATAATTTCCTGATGACGGGCCCCAAGGCCTATCTCATCTACTCCAGCAGTGTGGCTGCTGGGGCACAGAGTGGCATTGA GGAGTGCAAGTTCCAGTTTGCCTGGGATCGCTGGAACTGCCCCGAGCGGGCACTGCAGCTCTCCAGCCATGGCGGGCTG CGCAGTGCAAACCGAGAAACTGCCTTTGTCCATGCCATCAGCTCTGCAGGTGTCATGTACACGCTGACCCGGAACTGCA GCCTGGGAGATTTTGACAACTGTGGCTGTGATGACTCCCGCAATGGGCAGCTTGGTGGGCAAGGCTGGCTGTGGGGAGG CTGCAGCGATAATGTGGGCTTTGGGGAAGCCATCTCCAAGCAGTTTGTGGATGCCCTGGAGACTGGACAAGATGCCAGA GCTGCTATGAACCTGCACAACAACGAGGCAGGAAGAAAGGCTGTCAAAGGGACCATGAAGCGGACTTGCAAATGCCACG GTGTTTCAGGAAGTTGTACCACACAGACCTGCTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCACTTACCTCAAGGA GAGGTACCACAAGGCCTTGAAGGTGGACTTGCTGCAGGGGGTGGGGAACAGCGCTGCCAGCCGGGGGGCCATTGCCGAG ACCTTCAGCTCCATCTCCAAGAAGGAGCTGGTCCATTTAGAAGACTCTCCCGACTACTGCCTGGAGAACAAGACGCTGG GACTGCTGGGCACGGAGGGCCGCGAGTGTCTGAAGAGAGGCAAGGCGCTCAGCAAGTGGGAGAAGCGGAGCTGCCGGCG GCTGTGTGGGGACTGTGGGCTGGCGGTGGAGGAGAGACGTGCCGAGATGGTGTCCAGCTGCAACTGCAAGTTCCATTGG TGCTGCGCCGTGCGGTGTGAGCAGTGCCGCAAACGGGTCACCAAGTACTTCTGTGTCCGCAAAGAGAAGCGGGAGCAGA GCGGGGGTGGCGGGGCCAGCCGCAAGCTCAAGAGGAAGCTCTAA
Citation	Sienknecht UJ, Fekete DM. Mapping of Wnt, frizzled, and Wnt inhibitor gene expression domains in the avian otic primordium. J Comp Neurol. 2009 Dec 20;517(6):751-64.
Copyright	Copyright © (2002 Wiley-Liss); This material is used by permission of Wiley-Liss, Inc., a subsidiary of John Wiley & Sons, Inc.

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

19-20

stage 20

Ear/Otic Placode
Hindbrain

Entry 19

GEISHA Id	WNT8C.Stuhlmiller.2012	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Stuhlmiller TJ, García-Castro MI. FGF/MAPK signaling is required in the gastrula epiblast for avian neural crest induction. Development. 2012 Jan;139(2):289-300.
Copyright	© 2012. Published by The Company of Biologists Ltd
Comments	Insufficient information provided in publication to verify exact sequence used to synthesize probe. Sequence below was obtained from NCBI (acc # AB193181.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-5

stage 5

Primitive Streak

Entry 20

GEISHA Id	WNT8C.Vasiev.2010	Data Source	Publication
Complete cDNA Template Probe	show ATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCATGGTCGGTGA ATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAGTGGGATGGA GGAATGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCACAACCGCCTG CGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCAGGAACTGCA GCTTAGGAGACTTCGAGAGCTGCGGCTGCGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGTCTGGGGAGG CTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACACGATACCCGT GCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCAAGTGCCATG GGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTACCTGAAGAT GAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGCCGCGGCGCC ACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCACGAGGAACG CCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGAGCGGAGGAG CTGCCGGCGGCTCTGCACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGCAACTGCAAG TTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCCGCGATGCCG CCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAG
Citation	Vasiev B, Balter A, Chaplain M, Glazier JA, Weijer CJ. Modeling Gastrulation in the Chick Embryo: Formation of the Primitive Streak. PLoS One. 2010; 5(5): e10571.
Copyright	Copyright Vasiev et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI (acc #AB193181.1).

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

X-XIV

stage XIII

Kohler's Sickle

1-3

stage 2

Primitive Streak

stage 4

Early Mesoderm
Primitive Streak

Entry 21

GEISHA Id	WNT8C.Litsiou.2005	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Litsiou A, Hanson S, Streit A. A balance of FGF, BMP and WNT signalling positions the future placode territory in the head. Development. 2005 Sep;132(18):4051-62. Epub 2005 Aug 10
Copyright	Authors hold the copyright. Published by the Company of Biologists Ltd.
Comments	As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence below was obtained from NCBI NM_205531.1

Stage

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stage 7

Entry 22

GEISHA Id	cWnt-8c.Wittler.2008	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Wittler L, Saborowski M, Kessel M. Expression of the chick Sizzled gene in progenitors of the cardiac outflow tract. Gene Expr Patterns. 2008 Jul;8(6):471-6. Epub 2008 Feb 29.
Copyright	Copyright © 2008 Elsevier B.V.
Comments	The complete cDNA template sequence was obtained from the information from the publication as described in Wittler et al. 2008.

Stage

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4-5

stage 5

Early Endoderm
Early Mesoderm
Primitive Streak

Cresent, blue; Wnt8c, red
Szl, red; Wnt8c, blue

all

Entry 23

GEISHA Id	WNT8C.Lee.2020	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Hyung Chul Lee, Hui-Chun Lu, Mark Turmaine, Nidia M. M. Oliveira, Youwen Yang† , Irene De Almeida and Claudio D. Stern. Molecular anatomy of the pre-primitive streak chick embryo Open Biol. null. 10.1098/rsob.190299.
Copyright	Copyright © 2020 The Authors.
Comments	The complete cDNA template sequence was obtained from the information provided in the publication as described in Lee et al., 2020. CWNT-8C is also known as Wnt8c and WNT8A.

Stage

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Location (click to highlight)

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X-XIV

stage X

stage XII

stage XIV

Area Opaca
Epiblast
Hypoblast

GW, germinal wall
Stage EGK X-XI
Stage EGK XII-XIII
Stage EGK XIII-XIV

all

Entry 24

GEISHA Id	WNT8A.Olivera-Martinez.2014	Data Source	Publication
Complete cDNA Template Probe	show AATATCTGGAGATACGGCGTCAGTGCGGCAGCCCTGCCAGGGACCTGTGGGATCAGCAAAGGGAGCCAAGTTCCACGCT CTGACAGCCATGAGGGGCAGCACCTTCCTCCTCCTCTCCATCGTGGGCATCTACGGTGCTATTCTCAATGCAGCAGCAT GGTCGGTGAATAACTTTCTGATGACAGGACCTAAGGCGTACCTGACGTACTCCAGCAGCGTGGCGGCGGGCGCACAGAG TGGGATGGAGGAGTGCAAGTTCCAGTTCGGATGGGAGCGCTGGAACTGCCCCGAGAGCGCCCTGCAGCTCTCCACCCAC AACCGCCTGCGCAGTGCTACCAGAGAGACATCCTTTGTGCACGCCATCAGCTCCGCTGGTGTTATGTACACCCTGACCA GGAACTGCAGCTTAGGAGACTTCGAGAGCTGCGGCTGTGATGACTCCAGGAACGGGCGTGTAGGTGGCCGAGGCTGGGT CTGGGGAGGCTGCAGCGACAACGTGGAGTTTGGGGAGAGGATTTCCAAGCTCTTTGTGGATGCTTTGGAAACAGGACAC GATACCCGTGCCCTGATCAACCTGCACAACAACGAAGTCGGGAGACTTGCTGTGAAAGCCACGATGAAGCGAGCCTGCA AGTGCCATGGGGTGTCAGGCAGTTGCAGCATCCAGACATGCTGGCTCCAGCTCGCTGACTTTCGGGAGATTGGCAACTA CCTGAAGATGAAATACGACCAAGCGCATAAGCTGGAGATGGACAAGAGGCGGATGAGAGCTGGCAACAGCGCCGACAGC CGCGGCGCCACTGCTGAGACCTTCCACCACGTCCACTCCACGGAGCTCGTCTTTTTGGAGGACTCCCCCGACTACTGCA CGAGGAACGCCAGCCTGGGCCACCACGGCACCGAGGGCCGCGAGTGCCTACAGACCGGCAAGAACCTCTCTCAGTGGGA GCGGAGGAGCTGCCGGCGGCTCTCGACGGAGTGCGGCCTCAAAGTGGAGGAGAGGAGGACAGAGGTGGTCAGCAGCTGC AACTGCAAGTTCCACTGGTGCTGCACGGTGCGGTGCGAGCAGTGCCGGCAGCTGGTGGCCAAGCACTTCTGTGCCCGCC GCGATGCCGCCGTTGCCAACCACACCAAGCGGCGGAACAAAGGCCACAGGAGATAGGGGGAGGCTGGCAACCATGCCTG CCAGGCGGACCTGTGGGTTTCTTCACTTGTGGCGGCCCTCACCATGGCTGAGGTTGGGAAGGAGCCTTGCCCAGGGAAG TCACTTTATATCAGACAGAGGGAAGAATTTCACTTTTTCAGTGGGTTCCACGCATTGCACCACCGCCAGCCAAGGTCAA GCAGTGTTAGCATGAGTGGAACGGGCACCATGGCCGGGGCATGGTCCTGTTGGGCTGCTCCATTTCTGCGCAGGCACCA CAACCCCAGCATCCCTATTCCTGCACAGGCGTTGCTTGGAAGCACTCCTGATGGGGAAACTTGTGCACTTTCTGATGGG AGAGAGTTTTCTGAGTGCTGCTGTTTGTATTAGCACAGCTGCAGCTTTTTGAGGGCATCAGTAGGTTTTTAAGGACTGT GTTTACAGGGTTGATTTTACTTTTATAATGTTGTGGTTTGGTACCAGACCATCTACCCAAATGGATAACAGCTTCATGT GTCTGTACCGGGTGAAATTTAATTTGTTTACAATTCCTTTACTTTTTAAAAAACCAACGTAGCAAATACGAATTC
Citation	Isabel Olivera-Martinez, Nick Schurch, Roman A Li, Junfang Song, Pamela A Halley, Raman M Das, Dave W Burt, Geoffrey J Barton, Kate G Storey. Major transcriptome re-organisation and abrupt changes in signalling, cell cycle and chromatin regulation at neural differentiation in vivo. Development 2014 Aug;141(16):3266-76. Epub 2014 Jul 25. DOI: 10.1242/dev.112623.
Copyright	Copyright © 2014. Published by The Company of Biologists Ltd.
Comments	Authors indicated the probe was made from gene construct provided by J. Dodd. As insufficient information is provided in publication to verify exact sequence used to synthesize probe, the sequence above was obtained from NCBI (accession number NM_205531.1).

Stage

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Location (click to highlight)

Comments (click to highlight)

7-10

stage 10

Lateral Plate Mesoderm
Neural Plate/Tube
Primitive Streak

all

Data from NCBI Unigene EST Profile
Unigene ID: Gga.886

Mouse

Emage	MGI:107924
Eurexpress	MGI:107924
MGI GXD	MGI:107924

Frog

Xenbase

493705

Fruit Fly

BDGP

Zebra Finch

ZEBrA

Zebrafish

ZFIN	ZDB-GENE-980526-332

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WNT8AWnt family member 8A

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Entry 5

Entry 6

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Entry 10

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Entry 19

Entry 20

Entry 21

Entry 22

Entry 23

Entry 24

Mouse

Frog

Fruit Fly

Zebra Finch

Zebrafish

WNT8A
Wnt family member 8A