Sequence was obtained from NCBI (XM_416581). The primer pairs used were those given in the publication: 5'-GGAGCAGCATTCATCTTCTACAACG-3' as BOC-forward; and 5'-TGGTCACAGGCTTTGGTGGACTAC-3' as BOC-reverse.
The complete cDNA template sequence was obtained from the information provided in the publication as described in Singh et al. 2016. The probe was synthesized from cDNA clone ChEST870a16.