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A chicken embryo gene expression database

	Anatomical Location
	Stage
	Gene Name
	GEISHA ID
	Multiple Parameters

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MCF2L
MCF.2 cell line derived transforming sequence like

Gene

Official Symbol	MCF2L
Official Full Name	MCF.2 cell line derived transforming sequence like
BirdBase ID	BB-GG14176
CGNC ID	12632
also known as	guanine nucleotide exchange factor DBS
gene type	protein-coding

Genomic Map

	Ensembl
	NCBI
	UCSC
	Gallus-Browse

Sequence Information

Genomic
RNA	NM_001252063
Polypeptide	NP_001238992
Sequence Clusters/ESTs	Unigene

Gene Expression

In Situ Hybridization	GEISHA
EST Profile	Gga.12805

Orthology

	Entrez Gene	Ensembl Gene	Genetic Phenotypes	MOD
Fruit Fly
Human	23263	ENSG00000126217		MIM:609499
Mouse	17207	ENSMUSG00000031442	All phenotypic alleles (1):Targeted, other(1)	MGI:103263
Xenopus	100497730			6042030
Zebrafish	100148439	ENSDARG00000075859	ZDB-GENE-090312-122, ZDB-GENE-081104-416	ZFIN:ZDB-GENE-090312-122, ZFIN:ZDB-GENE-081104-416

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Links to other databases

Entrez Gene	418748
Ensembl Gene	ENSGALG00000016834
KEGG	gga:418748

Entry 1

GEISHA Id	MCF2L.UApcr	Data Source	GEISHA ISH Analysis
Forward cDNA Template Primer	GGTTAGCAGTAGAACAGGGAGACG
Reverse cDNA Template Primer	ATTAACCCTCACTAAAGGGAGATTTTACAAAGGCTTCGGGTC	cDNA Template Length	879
Forward cDNA Template Probe	show TTAGGTGCACTGGCTGAACAGGGACGTGTTTGCTTAGCTGCCAGTTTCTTAAGACTTACTTGTCTCTTTTGAAACATTT CTTCCATGCTTTCTTGCTTCTGGAAAACCTTTTGTATATGCTCCTTTAGGTCCTCATTGAGGATGTGTGCATACTCTCT GTAAACTTTATTCAACTCCTTAATTTTATTGCCTGCACCAGTGTCTAGGAATTTCTCAATCTCCTGTAATGCTGATTCT GCACCATCCTGAGACTGACACTTATCTACAGGTTGTGAAGCCAGAAGATAAATTCCTTCATCGCACCACTTCATAGACT TTTCCAGTAAGCCATGCAGTTCTAGAGATTTATTAAGAACATTCCTCCTCTGTTCCATCTCAGTAGTGAAGGCATCACA TAGATGATGAAGCTCACTGCATTTTGGAATAATGGAATCCACAGCATAATGATTGCTCTGAATAAAAGCATCACCCTCT GAAGCTAGCATTCTGGCTTTTGCTACAGTTTCACATGATTTTTC

Stage

Image (click image to view full size)

Location (click to highlight)

Comments (click to highlight)

4-6

stage 4

stage 5

stage 6

Area Pellucida
Extraembryonic Mesoderm
Hensen's Node
Neural Plate/Tube
Paraxial Mesoderm
Primitive Streak

7-12

stage 7

stage 10

stage 11

stage 12

Anterior Neuropore
Blood Islands
Extraembryonic Mesoderm
Head Mesenchyme
Hensen's Node
Intermediate Mesoderm
Lateral Plate Mesoderm
Neural Plate/Tube
Notochord
Paraxial Mesoderm
Primitive Streak
Somites

13-18

stage 13

stage 16

Blood Islands
Eye
Face Mesenchyme
Forebrain
Head Mesenchyme
Hindbrain
Intermediate Mesoderm
Lung
Midbrain
Myotome
Neural Plate/Tube
Notochord
Somites

19-24

stage 23

Forebrain
Gut
Head Mesenchyme
Midbrain
Somatic Mesoderm
Somites
Trunk Mesenchyme

stage 25

Eye
Forebrain
Head Mesenchyme
Midbrain
Notochord
Somites
Spinal Cord
Trunk Mesenchyme

Entry 2

GEISHA Id	MCF2L.Anderson.2022	Data Source	GEISHA ISH Analysis
Complete cDNA Template Probe	show TAAAAGATCTGTATGTCAAAGGCAATCTGTAAATCAAGGTCATCGCCCTTGTCCAGGTCCAAATTTGTTGGAGATGGAT TGATTGTCTTAATTTTCTTCCAATAAACAGTCCTTCAAGGAACAACATCAGAAACGTGAAAAAAATGGATGAGAGAAAA GCTGATCTCACAAGTCTAGAAGGTTATGTCACCACAGTAGCACCAAAGTACCCCGAGAAAGGCAAAGGTTGGGCTAAAA CGTCTCATTCTCTTGATGCATCTGAAGAAAATGATGGCTGGTCCAGTGCTGAGGAGCCGCTGAATTCATCGGATGCAGA AGAAGAAGGAGGAGGAGGAGGAGAGAGAAATGAGATGAAGCTGACTCCTGGTAAATATACAGTTGTGTCTGACTACGAG AAAGGAGTTTCTGAAGAATTTGCAGTGAAAGGTGGAGATCTAGTTCAACTGATTCGTGAAGGGGAGGAGGGACTTTGGT ATGTGAAGAATCTGACCACTGGTAGAGAAGGTTGGATCCCAGCAAACAATCTGCTGATGCTCATAGGCAATTCAAAATC AGCTCAATCTTTGAGCAGCTCTGAATCAGGCACTGCTTCCAGCACTTTGAGCACGTCATCAAGTTGCAGTGATAGCTGC AATGCCAGCAGCACCAGCTTCTCAGACATTAAGGGCTAATGTCATATGTTCACCCCACCTCCACGGAAAGTAAATCTGG AGTTTGAGCCCAACATCCAAAACTCGAGATGGCCATCAGAGAACTATCTGTTCTGAATTGCTGGTTTTTCAGGTGTTTT CTCTGATTTTCAGAGATTCTCACAGAAGATCCTTATGCAAAAGTGCTTGAATG
Citation	Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 Aug 25;7:12656. doi: 10.1038/ncomms12656.
Copyright	Copyright © 2016, The Author(s)
Comments	Images and probe sequence provided by Claire Anderson. The probe was synthesized from cDNA clone ChEST103i7. Related paper: Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA, Tickle C, Burt DW, Stern CD. A strategy to discover new organizers identifies a putative heart organizer. Nat Commun. 2016 7:12656. doi: 10.1038/ncomms12656.